DLG1 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: DLG1 Antibody [NBP3-38560] - Immunofluorescence analysis of L929 cells using DLG1 Rabbit pAb at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western Blot: DLG1 Antibody [NBP3-38560] - Western blot analysis of various lysates using DLG1 Rabbit pAb at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

DLG1 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DLG1 Antibody - BSA Free (NBP3-38560) is a polyclonal antibody validated for use in WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human DLG1 (NP_004078.2).
  
  Sequence:
  MPVRKQDTQRALHLLEEYRSKLSQTEDRQLRSSIERVINIFQSNLFQALIDIQEFYEVTLLDNPKCIDRSKPSEPIQPVNTWEISSLPSSTVTSETLPSSLSPSVEKYRYQDEDTPPQEHISPQITNEVIGPELVHVSEKNLSEIENVHGFVSHSHISPIKPTEAVLPSPPTVPVIPVLPVPAENTVILP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLG1
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:200
  • Western Blot 1:500 - 1:2000
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for DLG1 Antibody - BSA Free

• discs, large homolog 1 (Drosophila)
• disks large homolog 1
• DKFZp761P0818
• DKFZp781B0426
• hDlg
• large (Drosophila) homolog 1
• presynaptic protein SAP97

Background

DLG1(disks large homolog 1), also known as SAP97(synapse-associated protein 97), is a scaffolding protein that contains three PDZ domains, one SH3 domain, one L27 domain, and one guanylate kinase-like domain. DLG1has been found to control ion channel clustering at post-synaptic densities in neural cells and to also play a role in adherens junction assembly, and the recruitment of signaling molecules to discreet plasma membrane domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLG1