DLG1 Antibody

Western Blot: DLG1 Antibody [NBP1-26611] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Affinity purified rabbit anti-SAP97 used for WB at 0.04 mcg/ml (A) and 0.4 mcg/ml (B) and used for IP at 10 mcg/mg lysate. SAP97 was also immunoprecipitated by another rabbit anti-SAP97 antibody, which recognizes a downstream epitope.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DLG1 Antibody Summary

• Description: Novus Biologicals Rabbit DLG1 Antibody (NBP1-26611) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 50 and 100 of human synapse-associated protein 97 (discs, large homolog 1) using the numbering given in entry NP_004078.1 (GeneID 1739 ).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DLG1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 5-10 ug/mg lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DLG1 Antibody

• discs, large homolog 1 (Drosophila)
• disks large homolog 1
• DKFZp761P0818
• DKFZp781B0426
• hDlg
• large (Drosophila) homolog 1
• presynaptic protein SAP97

Background

SAP97 (synapse-associated protein 97), also known as DLG1 (disks large homolog 1), is a scaffolding protein that contains three PDZ domains, one SH3 domain, one L27 domain, and one guanylate kinase-like domain. SAP97 has been found to control ion channel clustering at post-synaptic densities in neural cells and to also play a role in adherens junction assembly, and the recruitment of signaling molecules to discreet plasma membrane domains.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DLG1
• Entrez:
  • 1739(Human)
• Uniprot:
  • Q12959(Human)