DKC1 Antibody (JU34-32)

Western Blot: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of DKC1 on A431 cell lysates with Rabbit anti-DKC1 antibody at 1/500 dilution. Lysates/proteins at 10 ug/Lane. Predicted band size: 58 kDa Observed band size: 58 kDa Exposure time: 1 minute; 10% SDS-PAGE gel. Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody at 1/500 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody at 1:200,000 dilution was used for 1 hour at room temperature.

Immunocytochemistry/Immunofluorescence: DKC1 Antibody (JU34-32) [NBP2-75472] - Staining DKC1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemistry-Paraffin: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of paraffin-embedded mouse brain tissue with Rabbit anti-DKC1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Western Blot: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of DKC1 on A431 cell lysate using anti-DKC1 antibody at 1/500 dilution.

Immunohistochemistry-Paraffin: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of paraffin-embedded human kidney tissue using anti-DKC1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of paraffin-embedded mouse testis tissue using anti-DKC1 antibody. Counter stained with hematoxylin.

Immunohistochemistry-Paraffin: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of paraffin-embedded rat brain tissue using anti-DKC1 antibody. Counter stained with

Immunohistochemistry-Paraffin: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of paraffin-embedded mouse kidney tissue using DKC1 antibody. Image from verified customer review.

Immunohistochemistry-Paraffin: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of paraffin-embedded human kidney tissue with Rabbit anti-DKC1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of paraffin-embedded mouse testis tissue with Rabbit anti-DKC1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of paraffin-embedded rat brain tissue with Rabbit anti-DKC1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Immunohistochemistry-Paraffin: DKC1 Antibody (JU34-32) [NBP2-75472] - Analysis of paraffin-embedded mouse hippocampus tissue with Rabbit anti-DKC1 antibody washed with ddH2O and PBS, and then probed with the primary antibody at 1/400 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Product Discontinued

DKC1 Antibody (JU34-32) Summary

• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: Synthetic peptide within Human DKC1 aa 353-402 / 514. (SwissProt: O60832 Human; SwissProt: Q9ESX5 Mouse; SwissProt: P40615 Rat)
• Localization: Cytoplasm. Nucleus.
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: DKC1
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/ Immunofluorescence 1:50-1:200
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:100-1:400
  • Western Blot 1:500-1:1000
• Theoretical MW: 57 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: TBS (pH7.4), 0.05% BSA, 40% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for DKC1 Antibody (JU34-32)

• CBF5 homolog
• CBF5
• cbf5p homolog
• DKC
• dyskeratosis congenita 1, dyskerin
• Dyskerin
• EC 5.4.99
• EC 5.4.99.-
• FLJ97620
• H/ACA ribonucleoprotein complex subunit 4
• NAP57
• NOLA4dyskerin
• Nopp140-associated protein of 57 kDa
• Nucleolar protein family A member 4
• Nucleolar protein NAP57
• snoRNP protein DKC1
• XAP101

Background

DKC1 is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the NOLA1, 2 and 3 proteins. The protein encoded by this gene and the three NOLA proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. These four H/ACA snoRNP proteins are also components of the telomerase complex. The protein encoded by this gene is related to the Saccharomyces cerevisiae Cbf5p and Drosophila melanogaster Nop60B proteins. The gene lies in a tail-to-tail orientation with the palmitoylated erythrocyte membrane protein gene and is transcribed in a telomere to centromere direction. Both nucleotide substitutions and single trinucleotide repeat polymorphisms have been found in this gene. Mutations in this gene cause X-linked dyskeratosis congenita, a disease resulting in reticulate skin pigmentation, mucosal leukoplakia, nail dystrophy, and progressive bone marrow failure in most cases. Mutations in this gene also cause Hoyeraal-Hreidarsson syndrome, which is a more severe form of dyskeratosis congenita. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for DKC1 Antibody (NBP2-75472) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Mouse.

reviewed by: Verified Customer IHC-P Mouse 03/05/2022

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: Kidney tissue
• Species: Mouse
• Lot: JU34-32

Bioinformatics

• Gene Symbol: DKC1