DISP1 Antibody

Immunocytochemistry/Immunofluorescence: DISP1 Antibody [NB100-1408] - (2.5ug/ml) staining of U87. Detected by mmunofluorescence. Data provided by Dr. Aashir Awan, University of Madrid, Spain.

Flow Cytometry: DISP1 Antibody [NB100-1408] - Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu, Mu, Rt, Po
• Applications: ELISA, Flow, ICC/IF
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DISP1 Antibody Summary

• Description: Novus Biologicals Goat DISP1 Antibody (NB100-1408) is a polyclonal antibody validated for use in ELISA, Flow and ICC/IF. Anti-DISP1 Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-SELSGESLLIKTL corresponding to C-Terminus according to NP_116279.2.
• Predicted Species: Mouse (100%), Rat (100%), Porcine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DISP1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 ug/mL
  • Immunocytochemistry/ Immunofluorescence 1:10 - 1:2000
  • Peptide ELISA Detection limit 1:32000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DISP1 Antibody

• DISP1
• DISPA
• DISPAFLJ43740
• dispatched A
• dispatched homolog 1 (Drosophila)
• Dispatched
• DKFZp434I0428
• MGC104180
• MGC13130
• MGC16796
• protein dispatched homolog 1

Background

The pattern of cellular proliferation and differentiation that leads to normal development of embryonic structuresoften depends upon the localized production of secreted protein signals. Cells surrounding the source of a particularsignal respond in a graded manner according to the effective concentration of the signal, and this response producesthe pattern of cell types constituting the mature structure. A novel segment-polarity gene known as dispatched hasbeen identified in Drosophila and its protein product is required for normal Hedgehog (Hh) signaling. This gene is oneof two human homologs of Drosophila dispatched and, based on sequence identity to its mouse counterpart, the encodedprotein may play an essential role in Hh patterning activities in the early embryo. (provided by RefSeq)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DISP1 Antibody (NB100-1408)(2)

Publications using NB100-1408

• Etheridge LA, Crawford TQ, Zhang S et al. Evidence for a role of vertebrate Disp1 in long-range Shh signaling. Development 2010-01-01 [PMID: 20023168]
• Burke R, Nellen D, Bellotto M et al. Dispatched, a novel sterol-sensing domain protein dedicated to the release of cholesterol-modified hedgehog from signaling cells. Cell 1999-12-23 [PMID: 10619433]

Bioinformatics

• Gene Symbol: DISP1
• Entrez:
  • 84976(Human)
• Uniprot:
  • NP_116279.(Human)