Rat DIO1 - Ready-To-Use ELISA Kit (Colorimetric)

• Reactivity: Rt
• Applications: ELISA
• Suitable Sample Type: Tissue homogenates and other biological fluids
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.059 ng/mL (example only; lot dependent)

Rat DIO1 - Ready-To-Use ELISA Kit (Colorimetric) Summary

• Description: The Ready-To-Use ELISA kit offers pre-diluted detection reagents and a shorter experimental time.
  Assay Length: 3 hours
• Standard Curve Range: 0.156 - 10 ng/mL (example only; lot dependent)
• Sensitivity: 0.059 ng/mL (example only; lot dependent)
• Assay Type: Sandwich ELISA
• Inter-Assay: %CV < 12 (example only; lot dependent)
• Intra-Assay: %CV < 10 (example only; lot dependent)
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: DIO1

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Rat DIO1 - Ready-To-Use ELISA Kit (Colorimetric)

• 5DI
• deiodinase, iodothyronine, type I
• DIOI
• EC 1.97.1
• EC 1.97.1.10
• ITDI1
• MGC130050
• thyroxine deiodinase type I (selenoprotein)
• TXDI1MGC130051
• Type 1 DI
• type I iodothyronine deiodinase
• Type-I 5'-deiodinase

Background

Dio-1 (Death Inducer-Obliterator-1) is a putative transcription factor that contains two zinc finger motifs. Dio-1 translocates to the nucleus, and activates apoptosis during limb development. Programmed cell death, a highly regulated form of apoptosis, plays an important role in determining the amount of tissue, the shape, and the definition of each digit during limb development. Dio-1 expression is upregulated when an apoptotic signal is detected, and subsequently apoptosis is induced. This process is similar to the expression of NFkappaB and NGF in response to external signals. Dio-1 expression is suppressed by caspase inhibitors and Bcl-2 expression. This supports the theory that Dio-1 functions in the onset of programmed cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for DIO1 ELISA Kit (NBP3-55207). (Showing 1 - 1 of 1 FAQ).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: DIO1