Dicer knockout Mouse embryonic stem cells

Images

 
Immunocytochemistry/ Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - SSEA1 antibody (NB100-1831) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei were ...read more
Immunohistochemistry: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - Brightfield Image of Dicer knock out Mouse embryonic stem cell line colonies growing directly on a gelatinized tissue culture flask.
Immunocytochemistry/ Immunofluorescence: Dicer knockout Mouse embryonic stem cells [NBP1-96751] - Nanog antibody (NB100-58842) was tested in Dicer knockout Mouse embryonic stem cells with DyLight 488 (green). Nuclei ...read more

Product Details

Summary

Order Details

Dicer knockout Mouse embryonic stem cells Summary

Specificity
Dicer knockout Mouse embryonic stem cells
Gene
DICER1

Applications/Dilutions

Application Notes
Please see Protocols and Procedures for culturing conditions.
Publications
Read Publications using
NBP1-96751 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store in gas phase of liquid nitrogen.
Buffer
Cells are supplied in 2 ml quantities (about 1x10 ^ 6 cells/ml) in freezing media (60% DMEM, 20% FBS, 20% DMSO)

Notes

Shipping is on dry ice. Proper long-term strorage is gas phase of liquid nitrogen.

Alternate Names for Dicer knockout Mouse embryonic stem cells

  • DCR1 ES cells
  • Dicer1 ES cells

Background

The Dicer knockout Mouse embryonic stem cells were derived from Dicer knockout mice. Dicer is essential for the processing of small non-coding RNAs including microRNAs and endogenous small interfering RNAs. These cells are a valuable research tool for scientists interested in studying the small non-coding RNAs in embryonic stem cells and the differentiated cells derived from them.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Support products are guaranteed for 6 months from date of receipt.

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Publications for Dicer knockout Mouse embryonic stem cells (NBP1-96751)(5)

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Product General Protocols

View specific protocols for Dicer knockout Mouse embryonic stem cells (NBP1-96751): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Dicer knockout Mouse embryonic stem cells (NBP1-96751). (Showing 1 - 3 of 3 FAQs).

  1. For NBP1-97434, the RIPA buffer contains SDS. Could you let us know the concentration of SDS in RIPA buffer?
    • The SDS is at 0.1%
  2. We recently ordered NBP1-96751. Which knock out mouse was this cell line generated? We went through the references but wasn't clear enough if the cell line is null/floxed or null/null.
    • This cell line was generated from Dicer flox/flox mice. I have provided two links below to publications referencing the production of this cell line. This is the most current information we have available from the inventor of the cell line. Please see NCBI Site or NCBI Site
  3. Are these cells prior to Cre treatment or are they cells that already have been Cre treated and have Dicer knocked out?
    • Our Dicer knockout mouse embryonic stem cells, NBP1-96751, are supplied treated and already have Dicer knocked out.

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Bioinformatics

Gene Symbol DICER1