Dicer knockout Mouse embryonic stem cells Lysate

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SDS-Page: Dicer knockout Mouse embryonic stem cells Whole Cell Lysate [NBP1-97435] - Staining of Dicer knockout Mouse embryonic stem cells Lysate using NBP1-97435.

Product Details

Summary
Reactivity MuSpecies Glossary
Applications WB, IP
Concentration
Please contact technical services for concentration.

Order Details

Dicer knockout Mouse embryonic stem cells Lysate Summary

Gene
DICER1

Applications/Dilutions

Application Notes
This lysate is prepared as a positive control for immunoprecipitation or separation by SDS-PAGE and subsequent Western blot analysis. Lysates are prepared in denaturing buffer WITHOUT loading buffer or reducing agents (i.e. 2-mercaptoethanol (BME) or dithiothreitol (DTT)). If reducing conditions are desired, add a reducing agent prior to heating. Heat lysate to 95 degrees C for 5 minutes and rapidly cool. The recommended loading amount is 0.01-0.03 mg per lane.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
Standard RIPA buffer including protease and phosphatase inhibitors and 0.1% SDS. No reducing agents or loading buffer added.
Preservative
No Preservative
Concentration
Please contact technical services for concentration.

Lysate Details for Array

Tissue
Embryo
Subcellular Fraction
Whole

Notes

Before use in Western blot, add sample loading buffer (Laemmli buffer) and reducing agent (BME or DTT).

Alternate Names for Dicer knockout Mouse embryonic stem cells Lysate

  • DCR1 ES cells
  • Dicer1 ES cells

Background

The Dicer knockout Mouse embryonic stem cells were derived from Dicer knockout mice. Dicer is essential for the processing of small non-coding RNAs including microRNAs and endogenous small interfering RNAs. These cells are a valuable research tool for scientists interested in studying the small non-coding RNAs in embryonic stem cells and the differentiated cells derived from them.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Other Available Formats

NBP1-97435

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Bioinformatics

Gene Symbol DICER1