DHX38 Antibody

Images

 
Immunohistochemistry: DHX38 Antibody [NB110-40582] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DHX38 used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB
Immunohistochemistry-Paraffin: DHX38 Antibody [NB110-40582] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-DHX38 used at a dilution of 1:250.
Immunohistochemistry-Paraffin: DHX38 Antibody [NB110-40582] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).
Immunoprecipitation: DHX38 Antibody [NB110-40582] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40582 used for IP at 3 mcg/mg lysate. DHX38 was also immunoprecipitated by NB110-40583, which ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications IHC, IHC-P, IP, WB (-)
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

DHX38 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residue 1150 and 1200 of human DEAH (Asp-Glu-Ala-His) Box Protein 38 using the numbering given in entry NP_054722.2 (GeneID 9785).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DHX38
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:500-1:2000
  • Immunoprecipitation 2-5 ug/mg
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DHX38 Antibody

  • ATP-dependent RNA helicase DHX38
  • DDX38pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38
  • DEAH (Asp-Glu-Ala-His) box polypeptide 38
  • DEAH box protein 38
  • EC 3.6.1
  • EC 3.6.4.13
  • KIAA0224pre-mRNA splicing factor ATP-dependent RNA helicase PRP16
  • PRP16 homolog of S.cerevisiae
  • PRP16hPrp16
  • PRPF16

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol DHX38
Entrez
Uniprot