DHX38 Antibody - BSA Free

Immunohistochemistry: DHX38 Antibody [NB110-40582] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti-DHX38 used at a dilution of 1:1,000 ( 1ug/ml). Detection: DAB

Immunohistochemistry-Paraffin: DHX38 Antibody [NB110-40582] - FFPE section of human breast carcinoma. Affinity purified rabbit anti-DHX38 used at a dilution of 1:250.

Immunohistochemistry-Paraffin: DHX38 Antibody [NB110-40582] - Human breast carcinoma. Antibody used at a dilution of 1:1000 (1ug/ml).

Immunoprecipitation: DHX38 Antibody [NB110-40582] - Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. NB110-40582 used for IP at 3 mcg/mg lysate. DHX38 was also immunoprecipitated by NB110-40583, which recognizes a downstream epitope.

• Reactivity: Hu
• Applications: IHC, IP, WB (-)
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1.0 mg/ml

DHX38 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit DHX38 Antibody - BSA Free (NB110-40582) is a polyclonal antibody validated for use in IHC and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: The immunogen recognized by this antibody maps to a region between residue 1150 and 1200 of human DEAH (Asp-Glu-Ala-His) Box Protein 38 using the numbering given in entry NP_054722.2 (GeneID 9785).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DHX38
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:500-1:2000
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 2-5 ug/mg lysate
• Application Notes: Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-Citrate/Phosphate (pH 7.0 - 8.0)
• Preservative: 0.09% Sodium Azide
• Concentration: 1.0 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DHX38 Antibody - BSA Free

• ATP-dependent RNA helicase DHX38
• DDX38pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 38
• DEAH (Asp-Glu-Ala-His) box polypeptide 38
• DEAH box protein 38
• EC 3.6.1
• EC 3.6.4.13
• KIAA0224pre-mRNA splicing factor ATP-dependent RNA helicase PRP16
• PRP16 homolog of S.cerevisiae
• PRP16hPrp16
• PRPF16

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. The protein encoded by this gene is a member of the DEAD/H box family of splicing factors. This protein resembles yeast Prp16 more closely than other DEAD/H family members. It is an ATPase and essential for the catalytic step II in pre-mRNA splicing process.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DHX38
• Entrez:
  • 9785(Human)
• Uniprot:
  • NP_054722.2(Human)