Recombinant Human DHRS4 His Protein

SDS-PAGE: Recombinant Human DHRS4 His Protein [NBP1-78825] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

SDS-PAGE: Recombinant Human DHRS4 His Protein [NBP1-78825] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.25 mg/ml

Recombinant Human DHRS4 His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-278 of Human DHRS4
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MGSHMHKAGL LGLCARAWNS VRMASSGMTR RDPLANKVAL VTASTDGIGF AIARRLAQDG AHVVVSSRKQ QNVDQAVATL QGEGLSVTGT VCHVGKAEDR ERLVATAVKL HGGIDILVSN AAVNPFFGSI MDVTEEVWDK TLDINVKAPA LMTKAVVPEM EKRGGGSVVI VSSIAAFSPS PGFSPYNVSK TALLGLTKTL AIELAPRNIR VNCLAPGLIK TSFSRMLWMD KEKEESMKET LRIRRLGEPE DCAGIVSFLC SEDASYITGE TVVVGGGTPS RL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DHRS4
• Purity: >85%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 32.1 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH7.5), 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.25 mg/ml
• Purity: >85%, by SDS-PAGE

Alternate Names for Recombinant Human DHRS4 His Protein

• CR
• dehydrogenase/reductase (SDR family) member 4 like 2A
• dehydrogenase/reductase (SDR family) member 4
• dehydrogenase/reductase SDR family member 4
• EC 1.1.1
• EC 1.1.1.184
• FLJ11008
• humNRDR
• NADP-dependent retinol dehydrogenase
• NRDRNADPH-dependent retinol dehydrogenase/reductase
• Peroxisomal short-chain alcohol dehydrogenase
• PHCR
• PSCD
• SCAD-SRLNADPH-dependent carbonyl reductase/NADP-retinol dehydrogenase
• SDR25C1
• SDR25C2
• SDR-SRL
• short chain dehydrogenase/reductase family 25C, member 1
• short chain dehydrogenase/reductase family 25C, member 2
• Short-chain dehydrogenase/reductase family member 4

Background

DHRS4, also known as dehydrogenase/reductase SDR family member 4, belongs to the short-chain dehydrogenases/reductases (SDR) family. DHRS4 reduces all trans retinal and 9 cis retinal. This protein can also catalyze the oxidation of all trans retinol with NADP as cofactor, but with much lower efficiency. Also, DHRS4 reduces alkyl phenyl ketones and alpha dicarbonyl compounds with aromatic rings, such as pyrimidine 4 aldehyde, 3 benzoylpyridine, 4 benzoylpyridine, menadione and 4 hexanoylpyridine. Recombinant human DHRS4 protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DHRS4