DGCR8 Antibody (4G3) - Azide and BSA Free

Western Blot: DGCR8 Antibody (4G3) [H00054487-M01] - DGCR8 monoclonal antibody (M01), clone 4G3. Analysis of DGCR8 expression in human spleen.

Immunohistochemistry-Paraffin: DGCR8 Antibody (4G3) [H00054487-M01] - Analysis of monoclonal antibody to DGCR8 on formalin-fixed paraffin-embedded human colon. Antibody concentration 6 ug/ml.

Western Blot: DGCR8 Antibody (4G3) [H00054487-M01] - Analysis of DGCR8 expression in transfected 293T cell line by DGCR8 monoclonal antibody (M01), clone 4G3.Lane 1: DGCR8 transfected lysate (Predicted MW: 86 KDa).Lane 2: Non-transfected lysate.

ELISA: DGCR8 Antibody (4G3) [H00054487-M01] - Detection limit for recombinant GST tagged DGCR8 is 0.1 ng/ml as a capture antibody.

• Reactivity: Hu
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 4G3
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free

DGCR8 Antibody (4G3) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse DGCR8 Antibody (4G3) - Azide and BSA Free (H00054487-M01) is a monoclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. Anti-DGCR8 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: DGCR8 (NP_073557, 675 a.a. ~ 771 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. LASQKILQLLHPHVKNWGSLLRMYGRESSKMVKQETSDKSVIELQQYAKKNKPNLHILSKLQEEMKRLAEEREETRKKPKMSIVASAQPGGEPLCTV
• Specificity: DGCR8 - DiGeorge syndrome critical region gene 8 (4G3)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DGCR8
• Purity: IgG purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Western Blot 1:500
• Application Notes: Antibody Reactive Against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: IgG purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DGCR8 Antibody (4G3) - Azide and BSA Free

• C22orf12
• DGCRK6chromosome 22 open reading frame 12
• DiGeorge syndrome critical region 8
• DiGeorge syndrome critical region gene 8
• Gy1
• microprocessor complex subunit DGCR8
• pasha

Background

DGCR8 is a component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis. Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-mi

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DGCR8 Antibody (H00054487-M01)(1)

Publication using H00054487-M01

• Nair-Menon J, Daulagala A, Connor D et al. Predominant Distribution of the RNAi Machinery at Apical Adherens Junctions in Colonic Epithelia Is Disrupted in Cancer. Int J Mol Sci. 2020-04-07 [PMID: 32272708]

Bioinformatics

• Gene Symbol: DGCR8
• Entrez:
  • 54487(Human)
• Uniprot:
  • NP_073557(Human)