DGAT2 Antibody

Product Discontinued

DGAT2 Antibody Summary

• Immunogen: A synthetic peptide made to an internal region of the human DGAT2 protein (within residues 100-200). [Swiss-Prot Q96PD7]
• Localization: Endoplasmic reticulum membrane; Multi-pass membrane protein
• Predicted Species: Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DGAT2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/ml
• Application Notes: This DGAT2 antibody is useful for Western blot, where a band is seen ~43 kDa.
• Control:

  Human Liver Whole Tissue Lysate (Adult Whole Normal)

Reactivity Notes

Human, yeast and mouse. Immunogen has 100% identity to rat, bovine and porcine, and 86% identity to Xenopus.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS and 30% Glycerol
• Preservative: 0.05% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DGAT2 Antibody

• DGAT2
• diacylglycerol O-acyltransferase 2
• diacylglycerol O-acyltransferase homolog 2 (mouse)
• diacylglycerol O-acyltransferase homolog 2
• diacylglycerol O-acyltransferase-like protein 2
• Diglyceride acyltransferase 2
• DKFZp686A15125
• EC 2.3.1
• EC 2.3.1.20
• GS1999FULL

Background

Diacylglycerol acyltransferase enzyme 2 is localized within the endoplasmic reticulum, mitochondria and lipid droplets. It catalyzes triacylglycerol synthesis, which creates the major form of stored energy in cells. In mice, when DGAT2 is overexpressed in the liver, hepatocytes have abnormally high levels of lipid retention, elevated triglyceride, diacylglycerol and ceramide, while maintaining a normal insulin response. This indicates that insulin resistance and elevated hepatic lipid retention are associated. DGAT2 is associated with increases in PKC activation, which is implicated in the formation of nonalcoholic fatty liver disease. DGAT2 is critical for lipid droplet formation in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DGAT2 Antibody (NBP1-71700)(5)

Publications using NBP1-71700

• McFie PJ, Chumala P, Katselis GS, Stone SJ DGAT2 stability is increased in response to DGAT1 inhibition in gene edited HepG2 cells Biochimica et biophysica acta. Molecular and cell biology of lipids 2021-09-01 [PMID: 34116261]
• Lee W, Koo HR, Choi YJ et al. Z-ligustilide and n-Butylidenephthalide Isolated from the Aerial Parts of Angelica tenuissima Inhibit Lipid Accumulation In Vitro and In Vivo Planta Med. 2019-05-28 [PMID: 31137047]
• Choi YJ, Lee CH, Lee KY et al. Increased Hepatic Fatty Acid Uptake and Esterification Contribute to Tetracycline-Induced Steatosis in Mice Toxicol. Sci. 2015-03-04 [PMID: 25745068] (WB, Human, Mouse)
  
  Details:
  DGAT2 antibody used for WB on lysates of liver tissues from mice administered or not with Tetracycline (FIG. 2A), whole cell lysates of HepG2 cells treated or not with Tetracycline, U0126 or PD98059 (FIG. 3C, 5A/B, Supplementary Figure 2B). Antibody also used for WB on lysates of HepG2 cells transfected with mock or HA tagged CA-MEK plasmid as well as on HepG2 cells subjected (FIG. 5A/B) or not to si-RNA mediated double knockdown of DGAT1/2 (FIG. 6A).
• Zeng T, Zhang CL, Song FY et al. CMZ Reversed Chronic Ethanol-Induced Disturbance of PPAR-a Possibly by Suppressing Oxidative Stress and PGC-1a Acetylation, and Activating the MAPK and GSK3b Pathway PLoS ONE. 2014-06-04 [PMID: 24892905] (WB, Mouse)
  
  Details:
  DGAT2 antibody used for WB in specific pathogen-free/SPF male Kun-Ming mice treated or not with chronic ethanol and/or chlormethiazole (CMZ). DGAT2 was studied as rate-limiting enzyme of G synthesis and its levels were found increased in ethanol and CMZ/ethanol group mice liver. (Figure 9A)
• Xu N, Zhang SO, Cole RA, McKinney SA, Guo F, Haas JT, Bobba S, Farese RV Jr, Mak HY. The FATP1-DGAT2 complex facilitates lipid droplet expansion at the ER-lipid droplet interface J Cell Biol 2012-08-27 [PMID: 22927462] (WB, Yeast)

Bioinformatics

• Gene Symbol: DGAT2
• Entrez:
  • 84649(Human)
  • 67800(Mouse)
• Uniprot:
  • Q96PD7(Human)