DGAT2 Antibody

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Western Blot: DGAT2 Antibody [NBP1-71700] - Analysis of DGAT2 in human liver lysate.

Product Details

Summary
Product Discontinued
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Order Details


    • Catalog Number
      NBP1-71700
    • Availability
      Product Discontinued

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DGAT2 Antibody Summary

Immunogen
A synthetic peptide made to an internal region of the human DGAT2 protein (within residues 100-200). [Swiss-Prot Q96PD7]
Localization
Endoplasmic reticulum membrane; Multi-pass membrane protein
Predicted Species
Rat (100%), Porcine (100%), Bovine (100%). Backed by our 100% Guarantee.
Clonality
Polyclonal
Host
Rabbit
Gene
DGAT2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 ug/ml
Application Notes
This DGAT2 antibody is useful for Western blot, where a band is seen ~43 kDa.
Positive Control
Liver Lysate (NB820-59233)
Publications
Read Publications using
NBP1-71700 in the following applications:

  • WB
    3 publications

Reactivity Notes

Human, yeast and mouse. Immunogen has 100% identity to rat, bovine and porcine, and 86% identity to Xenopus.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.05% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DGAT2 Antibody

  • DGAT2
  • diacylglycerol O-acyltransferase 2
  • diacylglycerol O-acyltransferase homolog 2 (mouse)
  • diacylglycerol O-acyltransferase homolog 2
  • diacylglycerol O-acyltransferase-like protein 2
  • Diglyceride acyltransferase 2
  • DKFZp686A15125
  • EC 2.3.1
  • EC 2.3.1.20
  • GS1999FULL

Background

Diacylglycerol acyltransferase enzyme 2 is localized within the endoplasmic reticulum, mitochondria and lipid droplets. It catalyzes triacylglycerol synthesis, which creates the major form of stored energy in cells. In mice, when DGAT2 is overexpressed in the liver, hepatocytes have abnormally high levels of lipid retention, elevated triglyceride, diacylglycerol and ceramide, while maintaining a normal insulin response. This indicates that insulin resistance and elevated hepatic lipid retention are associated. DGAT2 is associated with increases in PKC activation, which is implicated in the formation of nonalcoholic fatty liver disease. DGAT2 is critical for lipid droplet formation in adipocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DGAT2 Antibody (NBP1-71700)(3)

We have publications tested in 3 confirmed species: Human, Mouse, Yeast.

We have publications tested in 1 application: WB.


Filter By Application
WB
(3)
All Applications
Filter By Species
Human
(1)
Mouse
(2)
Yeast
(1)
All Species
Showing Publications 1 - 3 of 3.
Publications using NBP1-71700 Applications Species
Choi YJ, Lee CH, Lee KY et al. Increased Hepatic Fatty Acid Uptake and Esterification Contribute to Tetracycline-Induced Steatosis in Mice Toxicol. Sci. 2015 Mar 04 [PMID: 25745068] (WB, Human, Mouse)

Details:
DGAT2 antibody used for WB on lysates of liver tissues from mice administered or not with Tetracycline (FIG. 2A), whole cell lysates of HepG2 cells treated or not with Tetracycline, U0126 or PD98059 (FIG. 3C, 5A/B, Supplementary Figure 2B). Antibody also used for WB on lysates of HepG2 cells transfected with mock or HA tagged CA-MEK plasmid as well as on HepG2 cells subjected (FIG. 5A/B) or not to si-RNA mediated double knockdown of DGAT1/2 (FIG. 6A).
WB Human, Mouse
Zeng T, Zhang CL, Song FY et al. CMZ Reversed Chronic Ethanol-Induced Disturbance of PPAR-a Possibly by Suppressing Oxidative Stress and PGC-1a Acetylation, and Activating the MAPK and GSK3b Pathway PLoS ONE. 2014 Jun 04 [PMID: 24892905] (WB, Mouse)

Details:
DGAT2 antibody used for WB in specific pathogen-free/SPF male Kun-Ming mice treated or not with chronic ethanol and/or chlormethiazole (CMZ). DGAT2 was studied as rate-limiting enzyme of G synthesis and its levels were found increased in ethanol and CMZ/ethanol group mice liver. (Figure 9A)
WB Mouse
Xu N, Zhang SO, Cole RA, McKinney SA, Guo F, Haas JT, Bobba S, Farese RV Jr, Mak HY. The FATP1-DGAT2 complex facilitates lipid droplet expansion at the ER-lipid droplet interface J Cell Biol 2012 Aug 27 [PMID: 22927462] (WB, Yeast) WB Yeast

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Product General Protocols

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Video Protocols

WB Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Blogs on DGAT2.

Can you DGAT? DGAT1 & DGAT2 Antibodies for Metabolic Pathway Research
Triacylglycerols (triglycerides) (TGs) are the major storage molecules of metabolic energy and FAs in most living organisms. Excessive accumulation of TGs, however, is associated with human diseases, such as obesity, diabetes mellitus, and steatohepat...  Read full blog post.

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Bioinformatics

Gene Symbol DGAT2
Entrez
Uniprot