Mouse DFF40/CAD ELISA Kit (Colorimetric)

ELISA: Mouse DFF40/CAD ELISA Kit (Colorimetric) [NBP2-74966] - Samples were spiked with high concentrations of Mouse DFF40/CAD and diluted with Reference Standard & Sample Diluent to produce samples with values within the range of the assay.

ELISA: Mouse DFF40/CAD ELISA Kit (Colorimetric) [NBP2-74966] - Standard Curve Reference

• Reactivity: Mu
• Applications: ELISA
• Suitable Sample Type: Serum, plasma and other biological fluids
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL

Mouse DFF40/CAD ELISA Kit (Colorimetric) Summary

• Specificity: This kit recognizes Mouse CAD in samples. No significant cross-reactivity or interference between Mouse CAD and analogues was observed.
• Standard Curve Range: 1.56 - 100 ng/mL
• Sensitivity: 0.94 ng/mL
• Assay Type: Sandwich-ELISA
• Inter-Assay: CV% < 4.73%
• Intra-Assay: CV% < 4.43%
• Spike Recovery: 85-101%
• Sample Volume: 100 uL
• Kit Type: ELISA Kit (Colorimetric)
• Gene: DFFB

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Storage of components varies. See protocol for specific instructions.

Alternate Names for Mouse DFF40/CAD ELISA Kit (Colorimetric)

• CAD
• CADCaspase-activated nuclease
• Caspase-activated deoxyribonuclease
• Caspase-activated DNase
• CPAN
• CPANDNA fragmentation factor 40 kDa subunit
• DFF2
• DFF40
• DFF-40DFF40DFF2DNA fragmentation factor subunit beta
• DFFB
• DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase)
• EC 3.-

Background

Apoptosis is related to many diseases and induced by a family of cell death receptors and their ligands. Cell death signals are transduced by death domain containing adapter molecules and members of the caspase family of proteases. These death signals finally cause the degradation of chromosomal DNA by activated DNase. A mouse DNase that causes DNA fragmentation was identified recently and designated CAD (for caspase activated deoxyribonuclease). The human homologue of mouse CAD was more recently identified by two groups independently and termed CPAN and DFF40. Human DFF45 and its mouse homologue ICAD are the inhibitors of CPAN/DFF40 and CAD, respectively (1, 2, 5). Upon cleavage of DFF45/ICAD by activated caspase, DFF40/CAD is released and activated and eventually causes the degradation of DNA in the nuclei. Activation of CAD/DFF40, which causes DNA degradation, is the hallmark of apoptotic cell death.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. ELISA Kits are guaranteed for 6 months from date of receipt.

FAQs for DFF40/CAD ELISA Kit (NBP2-74966). (Showing 1 - 1 of 1 FAQ).

1. Does Novus offer any CAD (caspase activated DNase) antibodies that specifically look at the activation state of CAD? And if so, how well does this antibody work with chicken?
   • CAD/DFF40/DFFB is inhibited by being bound by DFFA. Because of this manner of inhibition, antibodies will not be able to recognize the difference between activated and inhibited DFFB, so we do not have a product specifically for activated or inhibited DFFB and this product will probably be nearly impossible to make. Unfortunately, the human and chicken sequence appear to be divergent for any of our immunogens to recommend any antibody to test for cross-reactivity with chicken. I apologize for the inconvenience.

Bioinformatics

• Gene Symbol: DFFB