Desmoplakin Antibody

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Immunohistochemistry-Paraffin: Desmoplakin Antibody [NBP1-49879] - Paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

Desmoplakin Antibody Summary

Immunogen
Peptide with sequence C-QESVKQAHLRNEH corresponding to internal region according to NP_004406.2.
Epitope
QESVKQAHLRNEH
Marker
Desmosomes Marker
Specificity
This antibody is expected to recognize reported isoform I (NP_004406.2) only.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
DSP
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100 - 1:2000
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:4000
Application Notes
WB: Preliminary experiments in lysates of HEK293, HeLa and NIH3T3 gave no specific signal but low background. IHC-P: Human heart shows staining of the junctons between cardiomuscular fibres.
Publications
Read Publication using NBP1-49879.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Mouse, Rat.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Desmoplakin Antibody

  • 250/210 kDa paraneoplastic pemphigus antigen
  • DCWHKTA
  • desmoplakin (DPI, DPII)
  • desmoplakin I
  • desmoplakin II
  • Desmoplakin
  • DP
  • DPI
  • DPII
  • DSP
  • KPPS2
  • PPKS2

Background

Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Desmoplakin Antibody (NBP1-49879)(1)

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Product General Protocols

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Video Protocols

WB Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DSP
Entrez
Uniprot