Desmoplakin Antibody

Immunohistochemistry-Paraffin: Desmoplakin Antibody [NBP1-49879] - Paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

Desmoplakin Antibody Summary

• Description: Novus Biologicals Goat Desmoplakin Antibody (NBP1-49879) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-Desmoplakin Antibody: Cited in 2 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-QESVKQAHLRNEH corresponding to internal region according to NP_004406.2.
• Epitope: QESVKQAHLRNEH
• Marker: Desmosomes Marker
• Specificity: This antibody is expected to recognize reported isoform I (NP_004406.2) only.
• Predicted Species: Mouse (100%), Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DSP
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 3 - 6 ug/ml
  • Immunohistochemistry-Paraffin 3 - 6 ug/ml
  • Peptide ELISA Detection limit 1:4000
  • Western Blot 1:100 - 1:2000
• Application Notes: WB: Preliminary experiments in lysates of HEK293, HeLa and NIH3T3 gave no specific signal but low background. IHC-P: Human heart shows staining of the junctons between cardiomuscular fibres.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for Desmoplakin Antibody

• 250/210 kDa paraneoplastic pemphigus antigen
• DCWHKTA
• desmoplakin (DPI, DPII)
• desmoplakin I
• desmoplakin II
• Desmoplakin
• DP
• DPI
• DPII
• DSP
• KPPS2
• PPKS2

Background

Major high molecular weight protein of desmosomes. Involved in the organization of the desmosomal cadherin-plakoglobin complexes into discrete plasma membrane domains and in the anchoring of intermediate filaments to the desmosomes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Desmoplakin Antibody (NBP1-49879)(2)

Publications using NBP1-49879

• Yilmaz O, Simsek Y, Inan S et al. Significant Changes in Trans-Epithelial Barrier Proteins of Adenoid Tissue with Atopic Status in Children Turk Thorac J 2020-07-01 [PMID: 32687784] (IHC-P, Human)
• Barahona-Dussault C, Benito B, Campuzano O et al. Role of genetic testing in arrhythmogenic right ventricular cardiomyopathy/dysplasia. Clin Genet 2010-01-01 [PMID: 19863551]

Bioinformatics

• Gene Symbol: DSP
• Entrez:
  • 1832(Human)
  • 109620(Mouse)
  • 306871(Rat)
• Uniprot:
  • NP_004406.2(Human)