Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoglein‑2 Monoclonal Antibody ...read more
Desmoglein‑2 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Desmoglein‑2 Monoclonal Antibody (Catalog # MAB947) at 10 µg/mL for 3 hours at room temperature. ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Desmoglein‑2 at approximately 120 kDa (as indicated) using 20 µg/mL of ...read more
Mouse myeloma cell line NS0-derived recombinant human Desmoglein-2 Ala50-Gly608 (predicted) Accession # CAA81226
Detects human Desmoglein-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25‑50% cross‑reactivity with recombinant human (rh) Desmoglein-1 is observed and no cross-reactivity with rhDesmoglein-3 is observed.
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Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Reconstitute at 0.5 mg/mL in sterile PBS.
This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.
Alternate Names for Desmoglein-2 Antibody (141409) [Unconjugated]
Cadherin family member 5
Desmoglein-2 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein-2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).
Nollet, R. et al. (2000) J. Mol. Biol. 299:551.
Elias, P. et al. (2001) J. Cell Biol. 153:243.
Arnemann, J. et al. (1992) Genomics 13:484.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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