Desmoglein-2 Antibody (141409) [Unconjugated]

Images

 
Western blot shows lysates of A431 human epithelial carcinoma cell line and A549 human lung carcinoma cell line. PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Desmoglein‑2 Monoclonal Antibody ...read more
Desmoglein‑2 was detected in immersion fixed NHEK human normal epidermal keratinocytes using Mouse Anti-Human Desmoglein‑2 Monoclonal Antibody (Catalog # MAB947) at 10 µg/mL for 3 hours at room temperature. ...read more
Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for Desmoglein‑2 at approximately 120 kDa (as indicated) using 20 µg/mL of ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Simple Western, ICC
Clone
141409
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

Desmoglein-2 Antibody (141409) [Unconjugated] Summary

Immunogen
Mouse myeloma cell line NS0-derived recombinant human Desmoglein-2
Ala50-Gly608 (predicted)
Accession # CAA81226
Specificity
Detects human Desmoglein-2 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25‑50% cross‑reactivity with recombinant human (rh) Desmoglein-1 is observed and no cross-reactivity with rhDesmoglein-3 is observed.
Source
N/A
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DSG2
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 2 ug/mL
  • Simple Western 20 ug/mL
  • Immunocytochemistry 5-25 ug/mL
Application Notes
In Simple Western only 10-15 uL of the recommended dilution is used per data point.
Publications
Read Publication using
MAB947 in the following applications:

  • IHC
    1 publication

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Desmoglein-2 Antibody (141409) [Unconjugated]

  • ARVC10
  • ARVD10
  • Cadherin family member 5
  • CDHF5
  • CDHF5MGC117034
  • CMD1BB
  • desmoglein 2
  • Desmoglein2
  • Desmoglein-2
  • DSG2
  • HDGC
  • MGC117036
  • MGC117037

Background

Desmoglein-2 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-2 is a type I transmembrane glycoprotein of 1117 amino acid (aa) residues with a 23 aa signal peptide and a 25 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, desmogleins have R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tails of desmogleins interact with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the desmogleins with the intermediate filaments. Desmoglein-2 has been shown to be important in establishing cell-cell adhesion and function in epithelial cells. Desmoglein-2 was originally identified in colon carcinoma and colon, and was named HDGC (human desmoglein colon).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for Desmoglein-2 Antibody (MAB947)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: IHC.


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Video Protocols

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Bioinformatics

Gene Symbol DSG2
Entrez
Uniprot