Desmoglein-1 Antibody [Alexa Fluor® 488]

• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 488

Desmoglein-1 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant human Desmoglein‑1 extracellular domain
  Glu50-His545
  Accession # Q02413
• Specificity: Detects human Desmoglein‑1 in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross‑reactivity with recombinant human (rh) Desmoglein-2 is observed and less than 1% cross-reactivity with rhDesmoglein-3, rhDesmoglein-4, and recombinant
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

Notes

This product is provided under an agreement between Life Technologies Corporation and R&D Systems, Inc, and the manufacture, use, sale or import of this product is subject to one or more US patents and corresponding non-US equivalents, owned by Life Technologies Corporation and its affiliates. The purchase of this product conveys to the buyer the non-transferable right to use the purchased amount of the product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). The sale of this product is expressly conditioned on the buyer not using the product or its components (1) in manufacturing; (2) to provide a service, information, or data to an unaffiliated third party for payment; (3) for therapeutic, diagnostic or prophylactic purposes; (4) to resell, sell, or otherwise transfer this product or its components to any third party, or for any other commercial purpose. Life Technologies Corporation will not assert a claim against the buyer of the infringement of the above patents based on the manufacture, use or sale of a commercial product developed in research by the buyer in which this product or its components was employed, provided that neither this product nor any of its components was used in the manufacture of such product. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, Cell Analysis Business Unit, Business Development, 29851 Willow Creek Road, Eugene, OR 97402, Tel: (541) 465-8300. Fax: (541) 335-0354.

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Desmoglein-1 is one of three members of the desmoglein subfamily of calcium-dependent cadherin cell adhesion molecules. Together with desmocollins, another subfamily within the cadherin superfamily, the desmoglein isoforms form the adhesive components of desmosomes, the cell-cell adhesive structures that are found in epithelial cells. Human Desmoglein-1 is a type I transmembrane glycoprotein of 1049 amino acid (aa) residues with a 23 aa signal peptide and a 26 aa propeptide. It differs from other classic cadherins by having four instead of five cadherin repeat domains in its extracellular region, and a much larger cytoplasmic region containing five desmoglein repeat domains which share homology with the cadherin repeats. Instead of having the HAV adhesion motif found in type I cadherins, Desmoglein-1 has R/YAL as the adhesion motif on its amino-terminal cadherin repeat. The cytoplasmic tail of Desmoglein-1 interacts with desmoplakins, plakoglobin and plakophilins. In turn, these proteins link the Desmoglein-1 with the intermediate filaments. Desmoglein-1 has been shown to be important in establishing cell-cell adhesion and function in the epidermis. In the autoimmune skin disease pemphigus foliaceus, autoantibodies to Desmoglein-1 can cause the loss of keratinocyte adhesion resulting in blisters.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.