Deoxyguanosine kinase Overexpression Lysate (Native)

Images

 
Western Blot: Deoxyguanosine kinase Overexpression Lysate (Adult Normal) [NBL1-09856] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for Deoxyguanosine kinase.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

Deoxyguanosine kinase Overexpression Lysate (Native) Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC204891 and based on accession number NM_080916. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens deoxyguanosine kinase (DGUOK), nuclear gene encoding mitochondrial protein, transcript variant 1, mRNA.
Gene
DGUOK

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
27.9 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Deoxyguanosine kinase

Type
Overexpression
Protein State
Native

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for Deoxyguanosine kinase Overexpression Lysate (Native)

  • deoxyguanosine kinase
  • deoxyguanosine kinase, mitochondrial
  • DGK
  • dGKmitochondrial deoxyguanosine kinase
  • EC 2.7.1.113
  • MTDPS3

Background

In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by two deoxyribonucleoside kinases, cytosolic deoxycytidine kinase and mitochondrial deoxyguanosine kinase. The protein encoded by this gene is responsible for phosphorylation of purine deoxyribonucleosides in the mitochondrial matrix. In addition, this protein phosphorylates several purine deoxyribonucleoside analogs used in the treatment of lymphoproliferative disorders, and this phosphorylation is critical for the effectiveness of the analogs. Alternative splice variants encoding different protein isoforms have been described for this gene. [provided by RefSeq]. Transcript Variant: This variant (1) is the longest transcript and encodes the longest isoform (a). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-43639
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP2-20626
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-92505
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-14868
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-01763
Species: Hu, Mu
Applications: WB, Flow, IHC, IHC-P
NBP1-47659
Species: Hu
Applications: WB, IHC, IHC-P
NBP2-07799
Species: Hu
H00005554-R02
Species: Hu
H00002038-M01
Species: Hu
Applications: WB, ELISA, ICC/IF
NBP1-33633
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IP
NBP1-84168
Species: Hu
Applications: IHC, IHC-P
MAB6240
Species: Hu
Applications: WB, IHC
NBP1-85740
Species: Hu, Po
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-45397
Species: Hu
Applications: WB, IHC, IHC-P
H00171023-M05
Species: Hu
Applications: WB, ELISA
NBP1-87769
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-89827
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P, IF
NB100-55256
Species: Hu
Applications: WB, IHC-P, IP
NBP2-13918
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBL1-09856
Species: Hu
Applications: WB

Publications for Deoxyguanosine kinase Lysate (NBL1-09856) (0)

There are no publications for Deoxyguanosine kinase Lysate (NBL1-09856).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Deoxyguanosine kinase Lysate (NBL1-09856) (0)

There are no reviews for Deoxyguanosine kinase Lysate (NBL1-09856). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Deoxyguanosine kinase Lysate (NBL1-09856) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional Deoxyguanosine kinase Products

Bioinformatics Tool for Deoxyguanosine kinase Lysate (NBL1-09856)

Discover related pathways, diseases and genes to Deoxyguanosine kinase Lysate (NBL1-09856). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Deoxyguanosine kinase Lysate (NBL1-09856)

Discover more about diseases related to Deoxyguanosine kinase Lysate (NBL1-09856).
 

Pathways for Deoxyguanosine kinase Lysate (NBL1-09856)

View related products by pathway.

PTMs for Deoxyguanosine kinase Lysate (NBL1-09856)

Learn more about PTMs related to Deoxyguanosine kinase Lysate (NBL1-09856).

Blogs on Deoxyguanosine kinase

There are no specific blogs for Deoxyguanosine kinase, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Deoxyguanosine kinase Overexpression Lysate (Native) and receive a gift card or discount.

Bioinformatics

Gene Symbol DGUOK