Recombinant Human Deoxycytidylate deaminase Protein

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Western Blot: Deoxycytidylate deaminase Protein [NBP1-45280] - 15% SDS-PAGE (3ug)

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human Deoxycytidylate deaminase Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-178 of Human Deoxycytidylate deaminase

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MSEVSCKKRD DYLEWPEYFM AVAFLSAQRS KDPNSQVGAC IVNSENKIVG IGYNGMPNGC SDDVLPWRRT AENKLDTKYP YVCHAELNAI MNKNSTDVKG CSMYVALFPC NECAKLIIQA GIKEVIFMSD KYHDSDEATA ARLLFNMAGV TFRKFIPKCS KIVIDFDSIN SRPSQKLQ

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
DCTD
Purity
>90%, by SDS-PAGE

Applications/Dilutions

Theoretical MW
22.1 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH8.0) containing 0.1M NaCl, 1mM DTT, 20% glycerol
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>90%, by SDS-PAGE

Alternate Names for Recombinant Human Deoxycytidylate deaminase Protein

  • dCMP deaminaseEC 3.5.4.12
  • deoxycytidylate deaminase
  • MGC111062

Background

DCTD (deoxycytidylate deaminase), also known as dCMP deaminase, is a 178 amino acid allosteric enzyme that exists as a homohexamer and belongs to the cytidine and deoxycytidylate deaminase protein family. Using zinc as a cofactor, DCTD catalyzes the deamination of dCMP to dUMP, thereby producing the nucleotide substrate (dUMP) that is used by thymidylate synthase. Recombinant human DCTD protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Bioinformatics

Gene Symbol DCTD