Recombinant Human Defensin beta 1 Protein

Product Discontinued

Recombinant Human Defensin beta 1 Protein Summary

• Description: Recombinant bioactive protein containing 47 amino acids for Human Defensin beta 1
  
  

  Source:E. coli
  Amino Acid Sequence:(P60022)

• Preparation Method: Determined to be >98% pure by SDS-PAGE
• Details of Functionality: Determined by its ability to chemoattract CD34+ dendritic cells using a concentration range of 0.1-1 ug/ml.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DEFB1
• Purity: >98%, by SDS-PAGE
• Endotoxin Note: <0.1 ng/ug

Applications/Dilutions

• Dilutions:
  • Bioactivity
  • SDS-Page
• Theoretical MW: 5 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C.
• Buffer: Lyophilized from 20 mM PBS (pH 7.4), 130 mM NaCl
• Concentration: Lyoph
• Purity: >98%, by SDS-PAGE
• Reconstitution Instructions: Reconstitute in dH2O to a concentration of 0.1 - 1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4C for 1 week or -20C for long-term storage.

Alternate Names for Recombinant Human Defensin beta 1 Protein

• BD1
• BD-1
• beta-defensin 1
• beta-defensin-1
• DEFB-1
• DEFB101
• defensin, beta 1HBD-1
• hBD-1
• HBD1MGC51822

Background

The Defensin family are highly similar in their protein sequence and are microbicidal & cytotoxic peptides made by neutrophils. Beta Defensin-1 (BD-1) is an antimicrobial peptide having the resistance of epithelial surfaces to microbial colonization. BD-1 has close proximity to Defensinalpha-1 and has been implicated in the pathogenesis of cystic fibrosis. Skin of patients having atopic dermatitis patients and mycosis fungoides (non-lesional and lesional) show lower human BD-1 mRNA expression and higher human BD-2 and human BD-3 mRNA expression. Beta Defensin is highly expressed by epithelial cells. BD-1 may play a role in the pathogenesis of severe sepsis. Variation in human BD-1 contributes to asthma diagnosis, with apparent gender-specific effects. Human BD-3 is a dimer, while Human BD-1 and Human BD-2 are monomeric. Recombinant human Beta Defensin-1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 47 amino acids and having a molecular mass of 5 kDa.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Bioinformatics

• Gene Symbol: DEFB1