Defensin alpha 5 Antibody (972207) [Unconjugated]

Recombinant Human alpha -Defensin 5 protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha -Defensin 5 Monoclonal Antibody (Catalog # MAB10248) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human alpha -Defensin 5 Monoclonal Antibody (Catalog # MAB102481) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

• Reactivity: Hu
• Applications: ELISA
• Clone: 972207
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

Defensin alpha 5 Antibody (972207) [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human alpha -Defensin 5
  Thr64-Arg94
  Accession # Q01523
• Specificity: Detects human alpha -Defensin 5 in direct ELISAs.
• Source: N/A
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Defensin alpha 5 Antibody (972207) [Unconjugated]

• alpha-Defensin 5
• DEF5
• DEF5defensin, alpha 5, preproprotein
• DEFA5
• defensin 5
• Defensin alpha 5
• Defensin, alpha 5
• defensin, alpha 5, Paneth cell-specific
• defensin-5
• HD-5
• MGC129728

Background

Defensin alpha 5 (DEFA5), also known as human alpha defensin 5 (HD5), is a secreted antimicrobial protein encoded by the DEFA5 gene and a member of the Defensins family. Defensins are thought to kill microbes by permeabilizing their plasma membrane. DEFA5 is expressed in Paneth cells, which are secretory epithelial cells in the small intestine. Unlike other defensins, DEFA5 is stored in secretory vesicles as a propeptide, and is processed into different peptides with different potency and localization.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Uniprot:
  • Q01523()