DEF6 Antibody (OTI3F9)

Images

 
Western Blot: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY DEF6.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human endometrium tissue.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human pancreas tissue.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human lymphoma tissue.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human tonsil tissue.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human bladder tissue.
Immunohistochemistry: DEF6 Antibody (3F9) [NBP2-46456] - Analysis of Human breast tissue.

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, IHC
Clone
OTI3F9
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

Order Details

DEF6 Antibody (OTI3F9) Summary

Immunogen
Full length human recombinant protein of human DEF6(NP_071330) produced in HEK293T cell.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
DEF6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:150
  • Western Blot 1:2000
Theoretical MW
73.7 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.3), 1.0% BSA and 50% Glycerol
Preservative
0.02% Sodium Azide
Concentration
1 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DEF6 Antibody (OTI3F9)

  • DEF-6
  • differentially expressed in FDCP 6 homolog (mouse)
  • differentially expressed in FDCP 6 homolog
  • IBPdifferentially expressed in FDCP (mouse homolog) 6
  • IRF4-binding protein
  • SLAT
  • SWAP70L
  • SWAP-70-like adaptor protein of T cells

Background

DEF6, or IBP, is a guanine nucleotide exchange factor (GEF) for RAC (MIM 602048) and CDC42 (MIM 116952) that is highly expressed in B and T cells (Gupta et al., 2003 [PubMed 12923183]).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

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Bioinformatics

Gene Symbol DEF6