DDX6 Antibody


Western Blot: DDX6 Antibody [NB200-192] - Detection of Human and Mouse DDX6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-192] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DDX6 (left) and Lot 3 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: ...read more
Independent Antibodies: Immunoprecipitation: DDX6 Antibody [NB200-192] - Detection of human DDX6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) ...read more
Genetic Strategies: Knockdown Validated: DDX6 Antibody [NB200-192] - Western blot analysis of HNRNPK and DDX6 protein levels upon HNRNPK or DDX6 knockdown. Image collected and cropped by CiteAb from the following ...read more

Product Details

Reactivity Hu, Mu, Gp, Pm-OrSpecies Glossary
Applications WB, ICC/IF, IHC, IP, KD
1.0 mg/ml

DDX6 Antibody Summary

The immunogen recognized by this antibody maps to a region between residues 425 and the C-terminus (residue 483) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656).
Predicted Species
Orangutan (100%), Guinea Pig (100%). Backed by our 100% Guarantee.
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence 1:500 - 1:2-500
  • Immunohistochemistry 1:1000- 1:5000
  • Immunohistochemistry-Paraffin 1:1000- 1:5000
  • Immunoprecipitation 2-10 ug/mg lysate
  • Knockdown Validated
  • Western Blot 1:2000 - 1:10000
Application Notes
Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections. ICC/IF reactivity reported in (PMID: 24728989).
DDX6 Overexpression Lysate
Read Publications using
NB200-192 in the following applications:

  • 5 publications
  • IP
    2 publications
  • KD
    2 publications
  • PLA
    1 publication
  • WB
    2 publications

Packaging, Storage & Formulations

Store at 4C. Do not freeze.
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
0.09% Sodium Azide
1.0 mg/ml
Immunogen affinity purified

Alternate Names for DDX6 Antibody

  • ATP-dependent RNA helicase p54
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
  • DEAD box protein 6
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
  • EC 3.6.1
  • EC
  • FLJ36338
  • HLR2DEAD box-6
  • Oncogene RCK
  • probable ATP-dependent RNA helicase DDX6
  • RCKP54


DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to cell proliferation and carcinogenesis.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DDX6 Antibody (NB200-192)(24)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 5 applications: ICC/IF, IP, KD, PLA, WB.

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Showing Publications 1 - 10 of 24. Show All 24 Publications.
Publications using NB200-192 Applications Species
Perez-Perri JI, Ferring-Appel D, Huppertz I et al. The RNA-binding protein landscapes differ between mammalian organs and cultured cells Nature communications 2023-04-12 [PMID: 37045843] (PLA, Mouse) PLA Mouse
Cochard, A;Garcia-Jove Navarro, M;Piroska, L;Kashida, S;Kress, M;Weil, D;Gueroui, Z; RNA at the surface of phase-separated condensates impacts their size and number Biophysical journal [PMID: 35364105]
Zanotti S, Vanhauwaert S, Van Neste C, et al. MYCN-induced nucleolar stress drives an early senescence-like transcriptional program in hTERT-immortalized RPE cells Sci Rep 2021-07-15 [PMID: 34262099]
Choksupmanee O, Tangkijthavorn W, Hodge K et al. Specific Interaction of DDX6 with an RNA Hairpin in the 3'-UTR of the Dengue Genome Mediates G1 Phase Arrest Journal of Virology 2021-06-16 [PMID: 34132569]
Furtado GV, Yang J, Wu D et al. FOXO1 controls protein synthesis and transcript abundance of mutant polyglutamine proteins, preventing protein aggregation Human molecular genetics 2021-04-02 [PMID: 33822053] (ICC/IF, Mouse) ICC/IF Mouse
Damodaran AP, Courtheoux T, Watrin E, Prigent C Alteration of SC35 localization by transfection reagents Biochim Biophys Acta Mol Cell Res 2020-01-15 [PMID: 31953060] (ICC/IF, Human) ICC/IF Human
Di Stefano B, Luo EC, Haggerty C et al. The RNA Helicase DDX6 Controls Cellular Plasticity by Modulating P-Body Homeostasis Cell Stem Cell 2019-09-27 [PMID: 31588046] (WB, ICC/IF, IP, KD, Mouse, Human) WB, ICC/IF, IP, KD Mouse, Human
Li J, Chen Y, Xu X et al. HNRNPK maintains epidermal progenitor function through transcription of proliferation genes and degrading differentiation promoting mRNAs Nat Commun 2019-09-13 [PMID: 31519929] (IP, WB, KD, Human) IP, WB, KD Human
Kaehler C, Isensee J, Hucho T et al. 5-Fluorouracil affects assembly of stress granules based on RNA incorporation. Nucleic Acids Res. 2014-04-11 [PMID: 24728989] (ICC/IF, Human) ICC/IF Human
Kaehler C, Isensee J, Nonhoff U et al. Ataxin-2-like is a regulator of stress granules and processing bodies. PLoS One. 2012-11-27 [PMID: 23209657] (ICC/IF, Human) ICC/IF Human
Show All 24 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Control Lysate(s)

Secondary Antibodies


Isotype Controls

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Gene Symbol DDX6