DDX6 Antibody

Images

 
Western Blot: DDX6 Antibody [NB200-192] - Detection of Human and Mouse DDX6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: ...read more
Immunohistochemistry-Paraffin: DDX6 Antibody [NB200-192] - FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti-DDX6 (left) and Lot 3 (right) used at a dilution of 1:1,000 (1ug/ml). Detection: ...read more
Independent Antibodies: Western Blot: DDX6 Antibody [NB200-192] - Human DDX6 on HeLa whole cell lysate using NB200-192. DDX6 was also IPed using rabbit anti-DDX6 antibody NB200-191, but not with 'DDX6 Ab', which ...read more
Western Blot: DDX6 Antibody [NB200-192] - Whole cell lysate (15 and 50 ug) from NIH3T3 cells. Antibody used at 0.2 ug/ml.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml
Validated by:
 

Independent Antibodies

       

DDX6 Antibody Summary

Immunogen
The immunogen recognized by this antibody maps to a region between residues 425 and the C-terminus (residue 483) of human DEAD (Asp-Glu-Ala-Asp) box polypeptide 6 using the numbering given Swiss-Prot entry P26196 (GeneID 1656).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX6
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:5000-1:15000
  • Immunocytochemistry/Immunofluorescence 1:500 - 1:2-500
  • Immunohistochemistry 1:1000- 1:5000
  • Immunohistochemistry-Paraffin 1:1000- 1:5000
  • Immunoprecipitation 2-5 ug/mg lysate
Application Notes
Immunohistochemistry 1:1,000 to 1:5,000. Epitope retrieval with Tris-EDTA pH9.0 is recommended for FFPE tissue sections.
Positive Control
DDX6 Lysate (NBL1-09810)
Publications
Read Publications using
NB200-192 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for DDX6 Antibody

  • ATP-dependent RNA helicase p54
  • DEAD (Asp-Glu-Ala-Asp) box polypeptide 6
  • DEAD box protein 6
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 6 (RNA helicase, 54kD)
  • EC 3.6.1
  • EC 3.6.4.13
  • FLJ36338
  • HLR2DEAD box-6
  • Oncogene RCK
  • probable ATP-dependent RNA helicase DDX6
  • RCKP54

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein. It may contribute to cell proliferation and carcinogenesis.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB200-192
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP

Publications for DDX6 Antibody (NB200-192)(16)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 1 application: ICC/IF.


Filter By Application
ICC/IF
(2)
All Applications
Filter By Species
Human
(2)
All Species
Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NB200-192 Applications Species
Kaehler C, Isensee J, Hucho T et al. 5-Fluorouracil affects assembly of stress granules based on RNA incorporation. Nucleic Acids Res. 2014 Apr 11 [PMID: 24728989] (ICC/IF, Human) ICC/IF Human
Kaehler C, Isensee J, Nonhoff U et al. Ataxin-2-like is a regulator of stress granules and processing bodies PLoS One 2012 [PMID: 23209657] (ICC/IF, Human) ICC/IF Human
Thomas MG, Luchelli L, Pascual M et al. A monoclonal antibody against p53 cross-reacts with processing bodies. PLoS One 2012 [PMID: 22590546]
Akhrymuk I, Kulemzin SV, Frolova EI et al. Evasion of the innate immune response: the Old World alphavirus nsP2 protein induces rapid degradation of Rpb1, a catalytic subunit of RNA polymerase II. J Virol 2012 Jul [PMID: 22514352]
Huang L, Mollet S, Souquere S et al. Mitochondria associate with P-bodies and modulate microRNA-mediated RNA interference. J Biol Chem 2011 Jul [PMID: 21576251]
Chien HL, Liao CL, Lin YL et al. FUSE binding protein 1 interacts with untranslated regions of Japanese encephalitis virus RNA and negatively regulates viral replication. J Virol 2011 May [PMID: 21367899]
Bennasser Y, Chable-Bessia C, Triboulet R et al. Competition for XPO5 binding between Dicer mRNA, pre-miRNA and viral RNA regulates human Dicer levels. Nat Struct Mol Biol 2011 Mar [PMID: 21297638]
Taddeo et al. PNAS 106 (29):12139-12144. 2009 [PMID: 19584246]
Minshall et al. Mol.Biol.Cell 20 (9):2464-2472. 2009 [PMID: 19297524]
Zhai et al. Mol.Cell.Biol. 28 (24):7414-7426. 2008 [PMID: 18936174]
Show All 16 Publications.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol DDX6
Entrez
Uniprot