DDX21 Antibody

Immunoprecipitation:[NBP3-29001]-Detection of human DDX21 by western blot of immunoprecipitates. Samples: Whole cell lysate from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-DDX21 antibody used for IP at 6 µg per reaction. DDX21 was also immunoprecipitated by rabbit anti-DDX21 antibodies and. For blotting immunoprecipitated DDX21, was used at 1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

• Reactivity: Hu, Rt
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX21 Antibody Summary

• Description: Novus Biologicals Rabbit DDX21 Antibody (NBP3-29001) is a polyclonal antibody validated for use in WB and IP. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: between 725 and C-terminus
• Epitope: Residue 725 and the C-terminus (residue 783) of human DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21 using the numbering given in entry NP_004719.2 (GeneID 9188).
• Predicted Species: Rat (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX21
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 2 - 10 µg/mg lysate
  • Western Blot 1:2000 - 1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: Tris-buffered Saline 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Affinity purified

Alternate Names for DDX21 Antibody

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• DEAD box protein 21
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21
• DKFZp686F21172
• EC 3.6.1
• EC 3.6.4.13
• Gu protein
• GUA
• gu-alpha
• GURDB
• nucleolar RNA helicase 2
• Nucleolar RNA helicase Gu
• Nucleolar RNA helicase II
• RH II/Gu
• RH-II/GU
• RH-II/GuA
• RNA helicase II/Gu alpha

Background

DDX21 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. DDX21 has been shown to be required for the processing of 20S rRNA to 18S and has also been shown to be important for c-Jun transcriptional activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX21