DDX21 Antibody

Western Blot: DDX21 Antibody [NB100-1716] - Detection of Human DDX21 on HeLa whole cell lysate using NB100-1716. DDX21 was also immunoprecipitated using rabbit anti-DDX21 antibodies NB100-1717 and NB100-1718.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX21 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residues 1 and 50 of human DEAD/H (Asp-Glu-Ala-Asp/His) Box Polypeptide 21 using the numbering given in entry NP_004719.2 (GeneID 9188).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX21
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:2000-1:10000
• Control:

  DDX21 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX21 Antibody

• DEAD (Asp-Glu-Ala-Asp) box polypeptide 21
• DEAD box protein 21
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 21
• DKFZp686F21172
• EC 3.6.1
• EC 3.6.4.13
• Gu protein
• GUA
• gu-alpha
• GURDB
• nucleolar RNA helicase 2
• Nucleolar RNA helicase Gu
• Nucleolar RNA helicase II
• RH II/Gu
• RH-II/GU
• RH-II/GuA
• RNA helicase II/Gu alpha

Background

DDX21 is a member of the DEAD box family of proteins that possesses several conserved motifs which include the highly conserved DEAD (Asp-Glu-Ala-Asp) amino acid sequence motif. The major activity of DEAD box proteins is to function as ATP-dependent RNA helicases. As helicases, DEAD proteins play an important role in all aspects of RNA metabolism and function which include pre-mRNA splicing, RNA synthesis, RNA degradation, RNA export, RNA translation, RNA secondary structure formation, ribosome biogenesis, and the assembly of RNP complexes. Some members of the DEAD box proteins also exhibit functions involved in transcriptional regulation. DDX21 has been shown to be required for the processing of 20S rRNA to 18S and has also been shown to be important for c-Jun transcriptional activation. Alternate names for DDX21 include nucleolar RNA helicase 2, nucleolar RNA helicase II, nucleolar RNA helicase Gu, RH II/Gu, Gu-alpha, DEAD box protein 21, GUA, GURDB, RH-II/GU, RH-II/GuA, and DKFZp686F21172.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DDX21 Antibody (NB100-1716)(1)

Publication using NB100-1716

• McRae, E K S, Dupas, S J Et al. An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21. RNA 2020-01-01 [PMID: 31653714] (ICC/IF, Drosophila melanogaster)

Bioinformatics

• Gene Symbol: DDX21
• Entrez:
  • 9188(Human)
• Uniprot:
  • NP_004719.2(Human)