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DDX11 Antibody

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Western Blot: DDX11 Antibody [NBP2-92510] - Analysis of extracts of various cell lines, using DDX11 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.
Western Blot: DDX11 Antibody [NBP2-92510] - Analysis of extracts of various cell lines, using DDX11 at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

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DDX11 Antibody Summary

Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human DDX11 (NP_689651.1).
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
DDX11
Purity
Affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
PBS with 50% glycerol, pH7.3.
Preservative
0.02% Sodium Azide
Purity
Affinity purified

Alternate Names for DDX11 Antibody

  • CHL1CHL1-related helicase gene-1
  • CHL1-related protein 1
  • CHLR1MGC9335
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (CHL1-like helicase homolog, S.cerevisiae)
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11 (S.cerevisiae CHL1-likehelicase)
  • DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 11
  • DEAD/H box protein 11
  • EC 3.6.1
  • EC 3.6.1.23
  • EC 3.6.1.7
  • EC 3.6.4.13
  • hCHLR1
  • Keratinocyte growth factor-regulated gene 2 protein
  • KRG-2
  • KRG2CHL1-like helicase homolog
  • MGC133249
  • probable ATP-dependent RNA helicase DDX11

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Western Blot: FAM49B Antibody [NBP1-88582] - Analysis in human cell line A-431.
Immunocytochemistry/Immunofluorescence: FAM49B Antibody [NBP1-88582] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleus.
Immunohistochemistry-Paraffin: FAM49B Antibody [NBP1-88582] - Staining of human lymph node shows strong cytoplasmic positivity in germinal center cells and non-germinal center cells.
Species: Hu
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L1CAM Antibody
(UJ127.11)
NB100-2682
Western Blot: L1CAM Antibody (UJ127.11) [NB100-2682] - SUM159 cells were exposed to 20% or 1% O2 for 48 hours, whole cell lysates were loaded with 50 ug/lane. 10% SDS-PAGE. L1CAM Antibody (NB100-2682) primary antibody: 1:1000, 4C, overnight. Western blot image submitted by a verified customer review.
Immunocytochemistry/Immunofluorescence: L1CAM Antibody (UJ127.11) [NB100-2682] - The left panel (A) shows untreated Neuro2a cells and the right panel (B) shows Neuro2a cells that were serum starved then treated with 1mM cAMP overnight to induce axon growth. Cells were fixed in 4% paraformaldehyde for 10 minutes and then permeabilized for 5 minutes using 1X PBS + 0.05% Triton-X100. The cells were incubated with anti- NB100-2682 at 5 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.
Immunohistochemistry-Paraffin: L1CAM Antibody (UJ127.11) [NB100-2682] - Analysis of a FFPE tissue section of human brain using 1:200 dilution of L1CAM [UJ127.11] antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. Cytoplasmic and membrane staining as observed in the cerebral cortex
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NBP1-31883
Western Blot: BRIP1/FANCJ Antibody [NBP1-31883] - Whole cell extract (30 ug) was separated by 5% SDS-PAGE, and the membrane was blotted with FANCJ antibody [N1N2], N-term diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.
Immunocytochemistry/Immunofluorescence: BRIP1/FANCJ Antibody [NBP1-31883] - 293T cells were fixed in 2% paraformaldehyde/culture medium at 37 degrees C for 30 min. Green: BRIP1 protein stained by FANCJ antibody [N1N2], N-term diluted at 1:500. Blue: Hoechst 33343 staining.
Immunohistochemistry-Paraffin: BRIP1/FANCJ Antibody [NBP1-31883] - Paraffin-embedded SAS xenograft; antibody dilution 1:500.
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CHTF18 Antibody
NBP1-03346
Western Blot: CHTF18 Antibody [NBP1-03346] - Whole cell lysate from HeLa (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 mcg) cells. NBP1-03346 used for WB at 0.04 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate.
Immunohistochemistry-Paraffin: CHTF18 Antibody [NBP1-03346] - Human stomach carcinoma. Antibody used at a dilution of 1:200 (1ug/ml).
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Applications: WB, IHC, IHC-P, IP
Contactin-6
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Contactin-4 Antibody
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H00004782-M01
Western Blot: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody (M01), clone 1C12-2A2.Lane 1: NFIC transfected lysate(47.6 KDa).Lane 2: Non-transfected lysate.
Immunocytochemistry/Immunofluorescence: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on HeLa cell. Antibody concentration 10 ug/ml.
Immunohistochemistry-Paraffin: NFIC Antibody (1C12-2A2) [H00004782-M01] - Analysis of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. Antibody concentration 3 ug/ml.
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Eco1 293T Cell
Transient Overexpress ...
H00114799-T01
Lane 1: ESCO1 transfected lysate (19.03 KDa) Lane 2: Non-transfected lysate.
ESCO1 transfected lysate.
Species: Hu
KGF/FGF-7
[Unconjugated]
251-KG
KGF/FGF-7 [Unconjugated]
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XPD Antibody
NBP2-20923
Western Blot: XPD Antibody [NBP2-20923] - Sample (30 ug of whole cell lysate) A: IMR32 B: U87-MG 7. 5% SDS PAGE gel, diluted at 1:1000.
Immunocytochemistry/Immunofluorescence: XPD Antibody [NBP2-20923] - XPD antibody detects XPD protein at cytoplasm and nucleus by immunofluorescent analysis. Sample: Mock and treated HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min. Green: XPD stained by XPD antibody diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar= 10 um.
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PCNA Antibody
(PC10)
NB500-106
Simple Western: PCNA Antibody (PC10) [NB500-106] - Image shows a specific band for PCNA in 0.5 mg/mL of HeLa lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Immunohistochemistry: PCNA Antibody (PC10) [NB500-106] - Development of the germinal zones in the tree shrew neocortex. Immunofluorescence for PCNA (yellow) and DAPI staining (blue) on 30 um-cryosections of E32-P1 tree shrew neocortex. The top margin of the image corresponds to the transition zone SVZ/intermediate zone. Scale bars, 50 um. VZ, ventricular zone; SVZ, subventricular zone; iSVZ, inner SVZ; oSVZ, outer SVZ. Image collected and cropped by CiteAb from the following publication (http://journal.frontiersin.org/article/10.3389/fnana.2018.00029/full), licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: PCNA Antibody (PC10) [NB500-106] - Analysis of paraffin-embedded human colon carcinoma, showing nuclear localization.
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NCAM-1/CD56
Antibody (123C3.D5)
NBP2-15184
Immunohistochemistry-Paraffin: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - Formalin-fixed, paraffin-embedded human colon Ganglion stained with CD56 Monoclonal Antibody (123C3.D5)
Flow (Cell Surface): NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - A surface stain was performed on U-87 Cells with NCAM-1/CD56 Antibody (123C3) NBP2-33132B and a matched isotype control. Both antibodies were conjugated to biotin. Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature, followed by Streptavidin - R-Phycoerythrin Protein (2012-1000, Novus Biologicals).
Flow Cytometry: NCAM-1/CD56 Antibody (123C3.D5) [NBP2-15184] - analysis of CD56 on human monocytes using CD56 antibody at 1 ug/10^6 cells.
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NFIB Antibody
NBP1-81000
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Immunocytochemistry/Immunofluorescence: NFIB Antibody [NBP1-81000] - Staining of human cell line U-251 MG shows localization to nucleoplasm & nucleoli fibrillar center. Antibody staining is shown in green.
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NFIA Antibody
NBP1-81406
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Western Blot: NFIA Antibody [NBP1-81406] - Analysis in human cell line HeLa and human cell line CACO-2.
Western Blot: NFIA Antibody [NBP1-81406] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
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NFIX Antibody
NBP2-15039
Western Blot: NFIX Antibody [NBP2-15039] - Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with NFIX antibody [N3C3] diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.
Immunocytochemistry/Immunofluorescence: NFIX Antibody [NBP2-15039] - Nfix is mainly expressed by anti-inflammatory MPs. Percentage of F4/80+ MPs positive for Nfix in Tibialis Anterior muscles (TA) of WT mice injected by CTX at D2, D4 and D7, post-injury. Immunostaining for F4/80 (green), Nfix (red) and DAPI (blue) at D4 and D7 after CTX injection. Results are means +/- SEM of at least three independent experiments. Scale bar = 50 um. Image collected and cropped by CiteAb from the following publication (https://www.mdpi.com/2073-4409/9/3/708) licensed under a CC-BY licence.
Immunohistochemistry-Paraffin: NFIX Antibody [NBP2-15039] - Mouse hippocampus. NFIX stained by NFIX antibody [N3C3] diluted at 1:500.
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Cardiotrophin-1/CT-1
612-CD
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c-jun Antibody
(5B1)
NBP2-37576
Western Blot: c-jun Antibody (5B1) [NBP2-37576] - Analysis using c-Jun mouse mAb against NIH/3T3 (1) and Cos7 (2) cell lysate.
Immunocytochemistry/Immunofluorescence: c-jun Antibody (5B1) [NBP2-37576] - Analysis of PC-2 cells using c-Jun mouse mAb (green). Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: c-jun Antibody (5B1) [NBP2-37576] - Analysis of human rectum cancer tissues using c-Jun mouse mAb with DAB staining.
Species: Hu, Mu, Pm
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P
RTEL1 Antibody
NBP2-22360
Western Blot: RTEL1 Antibody [NBP2-22360] - WB analysis of RTEL1 in HeLa cell lysate.
Immunocytochemistry/Immunofluorescence: RTEL1 Antibody [NBP2-22360] - RTEL1 antibody was tested in Hela cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Western Blot: RTEL1 Antibody [NBP2-22360] - WB analysis of RTEL1 in mouse testis lysate.
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ERK1 Antibody (1E5)
NBP2-22203
Western Blot: ERK1 Antibody (1E5) [NBP2-22203] - Western blot analysis of whole cell lysates from (1) MCF7 (2) NIH3T3 cell lines using ERK1 antibody (clone 1E5) at 1:1000 dilution. The signal was developed using HRP labeled goat-anti Mouse secondary antibody with ECL based detection.
Immunocytochemistry/Immunofluorescence: ERK1 Antibody (1E5) [NBP2-22203] - Analysis of NIH/3T3 cells using ERK1 mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.
Immunohistochemistry-Paraffin: ERK1 Antibody (1E5) [NBP2-22203] - Immunohistochemical analysis of paraffin-embedded breast cancer tissues using ERK1 mouse mAb with DAB staining.
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Tyr307] Antibody [Unc ...
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Bioinformatics

Gene Symbol DDX11