DDX10 Antibody

Western Blot: DDX10 Antibody [NB100-1577] - Detection of Human DDX10 on HeLa whole cell lystate using NB100-1577. DDX10 was also immunoprecipitated with rabbit anti-DDX10 antibody NB100-1576.

• Reactivity: Hu
• Applications: WB, IP
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

DDX10 Antibody Summary

• Immunogen: The immunogen recognized by this antibody maps to a region between residue 825 and the C-terminus (residue 875) of human DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 10 using the numbering given in entry NP_004389.2 (GeneID 1662).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DDX10
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunoprecipitation 1-4 ug/mg of lysate
  • Western Blot 1:2000-1:10000

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: TBS and 0.1% BSA
• Preservative: 0.09% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DDX10 Antibody

• DDX10-NUP98 fusion protein type 2
• DEAD (Asp-Glu-Ala-Asp) box polypeptide 10
• DEAD box protein 10
• DEAD box-10
• DEAD/H (Asp-Glu-Ala-Asp/His) box polypeptide 10 (RNA helicase)
• EC 3.6.1
• EC 3.6.4.13
• HRH-J8
• probable ATP-dependent RNA helicase DDX10

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division. This gene encodes a DEAD box protein, and it may be involved in ribosome assembly. Fusion of this gene and the nucleoporin gene, NUP98, by inversion 11 (p15q22) chromosome translocation is found in the patients with de novo or therapy-related myeloid malignancies.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DDX10
• Entrez:
  • 1662(Human)
• Uniprot:
  • NP_004389.2(Human)