DDT Antibody [Alexa Fluor® 488]

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Alexa Fluor 488

DDT Antibody [Alexa Fluor® 488] Summary

• Immunogen: E. coli-derived recombinant mouse DDT
  Pro2-Leu118
  Accession # O35215
• Specificity: Detects mouse and rat DDT in direct ELISAs and Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DDT Antibody [Alexa Fluor® 488]

• DDCT
• D-Dopachrome Decarboxylase
• D-dopachrome tautomeraseDDCT
• DDT
• DOPD
• EC 4.1.1.84
• MIF2
• Phenylpyruvate Tautomerase II

Background

Mouse DDT (D-dopachrome tautomerase; also D-dopachrome decarboxylase and Phenylpyruvate tautomerase) is a 13 kDa member of the carboxy-lyases family. The DDT gene in mouse, rat and human is identical in exon structure to the Macrophage migration inhibitory factor (MIF) gene. Both genes have 2 introns that are located at equivalent positions, relative to a 2-fold repeat in protein structure. The genes for DDT and MIF are closely linked on human chromosome 22 and mouse chromosome 10. DDT mRNA levels in human adipocytes are negatively correlated with obesity. The study on DDT knock-down adipocytes shows an increase in the expression of genes involved in both lipolysis and lipogenesis. DDT binds CD74 with high affinity, leading to activation of ERK1/2 MAP kinase and downstream pro-inflammatory pathways. Circulating levels of DDT correlate with disease severity in sepsis or malignancy. DDT also inhibits chemotaxis induced by CCL2 in macrophages. DDT in tumor angiogenesis promotes the expression of pro-angiogenic factors CXCL8 (IL8) and VEGF-A in non-small cell lung carcinoma cells through the CD74 receptor.  Over aa 2-118, mouse DDT shares 95.7% and 73.5% aa identity with rat and human DDT, respectively.  

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.