DCUN1D1 Antibody

Western Blot: DCUN1D1 Antibody [NBP1-52025] - Analysis of DCUN1D1 in HepG2 lysate (35ug protein in RIPA buffer) using NBP1-52025 at 0.5 ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Hu, Mu, Bv
• Applications: WB, ELISA, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DCUN1D1 Antibody Summary

• Description: Novus Biologicals Goat DCUN1D1 Antibody (NBP1-52025) is a polyclonal antibody validated for use in IHC, WB and ELISA. Anti-DCUN1D1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-RPQIAGTKSTT corresponding to C-Terminus according to NP_065691.2.
• Epitope: C-RPQIAGTKSTT
• Predicted Species: Mouse (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DCUN1D1
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 5 - 10 ug/ml
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 0.3 - 1 ug/ml
• Application Notes: WB: Approx. 28 kDa band observed in HepG2 lysates (calculated MW of 30.1 kDa band according to NP_065691.2). IHC-P: Human kidney shows pixulated staining in the cytoplasm of epithelial cells of PCT, but heavy cytoplasm staining in the epithelial cells of the DCT.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DCUN1D1 Antibody

• DCN1
• DCN1, defective in cullin neddylation 1, domain containing 1 (S. cerevisiae)
• DCN1-like protein 1
• DCNL1
• DCUN1D1
• DCUN1L1
• DCUN1L1DCUN1 domain-containing protein 1
• Defective in cullin neddylation protein 1-like protein 1
• RP42 homolog
• RP42
• RP42Squamous cell carcinoma-related oncogene
• SCCRO
• SCCROSCRO
• Tes3

Background

DCUN1D1 may contribute to neddylation of cullin components of SCF-type E3 ubiquitin ligase complexes. Neddylation of cullins play an essential role in the regulation of SCF-typecomplexes activity

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DCUN1D1 Antibody (NBP1-52025)(1)

Publication using NBP1-52025

• Villa C, Venturelli E, Fenoglio C et al. DCUN1D1 is a risk factor for frontotemporal lobar degeneration. Eur J Neurol 2009-07-01 [PMID: 19473369]

Bioinformatics

• Gene Symbol: DCUN1D1
• Entrez:
  • 54165(Human)
  • 114893(Mouse)
• Uniprot:
  • NP_065691.2(Human)