DCPS Antibody (1G4)

Western Blot: DCPS Antibody (1G4) [H00028960-M02] - DCPS monoclonal antibody (M02), clone 1G4 Analysis of DCPS expression in HeLa.

Immunocytochemistry/Immunofluorescence: DCPS Antibody (1G4) [H00028960-M02] - Analysis of monoclonal antibody to DCPS on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry-Paraffin: DCPS Antibody (1G4) [H00028960-M02] - Analysis of monoclonal antibody to DCPS on formalin-fixed paraffin-embedded human kidney. Antibody concentration 1.5 ug/ml.

Western Blot: DCPS Antibody (1G4) [H00028960-M02] - DCPS monoclonal antibody (M02), clone 1G4. Analysis of DCPS expression in PC-12.

Sandwich ELISA: DCPS Antibody (1G4) [H00028960-M02] - Detection limit for recombinant GST tagged DCPS is approximately 3ng/ml as a capture antibody.

• Reactivity: Hu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clone: 1G4
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated

DCPS Antibody (1G4) Summary

• Immunogen: DCPS (AAH14532, 1 a.a. ~ 337 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MADAAPQLGKRKRELDVEEAHAASTEEKEAGVGNGTCAPVRLPFSGFRLQKVLRESARDKIIFLHGKVNEASEDGDGEDAVVILEKTPFQVEQVAQLLTGSPELQLQFSNDIYSTYHLFPPRQLNDVKTTVVYPATEKHLQKYLRQDLRLIRETGDDYRNITLPHLESQSLSIQWVYNILDKKAEADRIVFENPDPSDGFVLIPDLKWNQQQLDDLYLIAICHRRGIRSLRDLTPEHLPLLRNILHQGQEAILQRYRMKGDHLRVYLHYLPSYYHLHVHFTALGFEAPGSGVERAHLLAEVIENLECDPRHYQQRTLTFALRADDPLLKLLQEAQQS
• Specificity: DCPS (1G4)
• Isotype: IgG2b Kappa
• Clonality: Monoclonal
• Host: Mouse
• Gene: DCPS
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin
  • Sandwich ELISA
  • Western Blot 1:500
• Application Notes: Antibody reactive against cell lysate and recombinant protein for Western Blot. Has also been used for immunofluoresence, immunohistochemistry (paraffin), and ELISA.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: In 1x PBS, pH 7.4
• Preservative: No Preservative
• Purity: Protein A purified

Notes

This product is produced by and distributed for Abnova, a company based in Taiwan.

Alternate Names for DCPS Antibody (1G4)

• DCS1
• DCS-1
• decapping enzyme, scavenger
• EC 3.-
• heat shock-like protein 1
• HINT5
• HINT-5homolog of C. elegans 7meGMP-directed hydrolase dcs-1
• Hint-related 7meGMP-directed hydrolase
• Histidine triad protein member 5
• HSL1
• HSPC015
• mRNA decapping enzyme
• scavenger mRNA-decapping enzyme DcpS

Background

Necessary for the complete degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Removes the 7-methyl guanine cap structure from mRNA fragments shorter than 10 nucleotides that are produced by 3'->5' exosome-mediated mRNA decay. Releases m7GMP. Can also degrade m7GDP to m7GMP. Has no activity towards mRNA molecules longer than 25 nucleotides

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DCPS
• Entrez:
  • 28960(Human)
  • 69305(Mouse)
  • 266605(Rat)
• Uniprot:
  • AAH14532(Human)