![Western Blot: DCAF11 Antibody [NBP1-57691] - TFEB represses the expression of DCAF11. The protein levels of Keap1 and Nrf2-specific E3 ubiquitin ligases in H4 and TFEB cells were analyzed by immunoblotting using each corresponding antibody. Full blots are provided in Supplementary Fig. S10. Data shown are mean +/- S.E. of three independent experiments and were analyzed using Student's t test. (**p < 0.01; ***p < 0.001). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-50877-8) licensed under a CC-BY license.](http://images.novusbio.com/fullsize/DCAF11-Antibody-Western-Blot-NBP1-57691-img0006.jpg "Western Blot: DCAF11 Antibody [NBP1-57691] - TFEB represses the expression of DCAF11. The protein levels of Keap1 and Nrf2-specific E3 ubiquitin ligases in H4 and TFEB cells were analyzed by immunoblotting using each corresponding antibody. Full blots are provided in Supplementary Fig. S10. Data shown are mean +/- S.E. of three independent experiments and were analyzed using Student's t test. (**p < 0.01; ***p < 0.001). Image collected and cropped by CiteAb from the following publication (//www.nature.com/articles/s41598-019-50877-8) licensed under a CC-BY license.")
| Reactivity | HuSpecies Glossary |
| Applications | WB, IHC |
| Clonality | Polyclonal |
| Host | Rabbit |
| Conjugate | Unconjugated |
| Format | BSA Free |
| Concentration | 0.5 mg/ml |
| Description | The addition of 50% glycerol is optional for those storing this antibody at -20C and not aliquoting smaller units. However, please note that glycerol may interrupt some downstream antibody applications and should be added with caution. |
| Immunogen | Synthetic peptides corresponding to WDR23(WD repeat domain 23) The peptide sequence was selected from the N terminal of WDR23. Peptide sequence GSRNSSSAGSGSGDPSEGLPRRGAGLRRSEEEEEEDEDVDLAQVLAYLLR. The peptide sequence for this immunogen was taken from within the described region. |
| Isotype | IgG |
| Clonality | Polyclonal |
| Host | Rabbit |
| Gene | DCAF11 |
| Purity | Affinity purified |
| Innovator's Reward | Test in a species/application not listed above to receive a full credit towards a future purchase. |
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| Theoretical MW | 62 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors. |
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| Publications |
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| Storage | Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles. |
| Buffer | PBS, 2% Sucrose |
| Preservative | 0.09% Sodium Azide |
| Concentration | 0.5 mg/ml |
| Purity | Affinity purified |
| Publication using NBP1-57691 | Applications | Species |
|---|---|---|
| Park JY, Kim S, Sohn HY et al. TFEB activates Nrf2 by repressing its E3 ubiquitin ligase DCAF11 and promoting phosphorylation of p62 Sci Rep 2019-10-04 [PMID: 31586112] (WB, Human) | WB | Human |
Secondary Antibodies |
Isotype Controls |
Research Areas for DCAF11 Antibody (NBP1-57691)Find related products by research area.
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![Immunohistochemistry-Paraffin: DCAF11 Antibody [NBP1-57691] - Formalin Fixed Paraffin; Embedded Tissue: Human Adult Liver; Observed Staining: Cytoplasm in hepatocytes, strong signal, moderate tissue distribution; Primary Antibody Concentration: 1:100](http://images.novusbio.com/fullsize/DCAF11-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-57691-img0005.jpg "Immunohistochemistry-Paraffin: DCAF11 Antibody [NBP1-57691] - Formalin Fixed Paraffin; Embedded Tissue: Human Adult Liver; Observed Staining: Cytoplasm in hepatocytes, strong signal, moderate tissue distribution; Primary Antibody Concentration: 1:100")
![Western Blot: DCAF11 Antibody [NBP1-57691] - Titration: 0.2-1 ug/ml, Positive Control: Human Placenta.](http://images.novusbio.com/fullsize/DCAF11-Antibody-Western-Blot-NBP1-57691-img0003.jpg "Western Blot: DCAF11 Antibody [NBP1-57691] - Titration: 0.2-1 ug/ml, Positive Control: Human Placenta.")
![Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).](https://images.novusbio.com/images/Goat-anti-Rabbit-IgG-H+L-Secondary-Antibody-HRP-Western-Blot-NB7160-img0001.jpg "Western Blot: Goat anti-Rabbit IgG (H+L) Secondary Antibody [HRP] [NB7160] - Western blot showing vemurafenib treatment in BRAFV600E CRC cells inhibits fission mediator DRP1 with no significant effect on fusion proteins (Mfn1 & 2) using MFN-1 antibody (NBP1-51841) and corresponding secondary antibody, goat anti-rabbit IgG-HRP (NB7160). Image collected and cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33738242).")
![Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.](https://images.novusbio.com/images/Rabbit--Mouse-IgG-Isotype-Control-Flow-Cytometry-NBP2-24891-img0001.jpg "Flow Cytometry: Rabbit IgG Isotype Control [NBP2-24891] - Intracellular FACS analysis of mouse TLR6 polyclonal antibody (red), rabbit isotype control (green), RAW cells alone (shaded). Two micrograms of antibodies were used. Goat anti-rabbit FITC (Novus, 20302) was used as secondary.")