DC-SIGNR/CD299/CLEC4M Overexpression Lysate

Western Blot: CLEC4M Overexpression Lysate (Adult Normal) [NBL1-09263] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CLEC4M.

• Reactivity: Hu
• Applications: WB

DC-SIGNR/CD299/CLEC4M Overexpression Lysate Summary

• Description:

  DC-SIGNR/CD299/CLEC4M Transient Overexpression Lysate

  
  Expression Host: HEK293T
  
  Plasmid: RC211716
  
  Accession#: NM_214675
  
  Protein Tag: C-MYC/DDK
  
  You will receive 1 vial of lysate (100ug), 1 vial of empty vector negative control (100ug), and 1 vial of 2xSDS sample buffer (250ul). Each vial of cell lysate contains 100ug of total protein (at 1 mg/ml). The 2xSDS Sample Buffer consists of 4% SDS, 125mM Tris-HCl pH6.8, 10% Glycerol, 0.002% Bromophenol blue, 100mM DTT.
• Gene: CLEC4M

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This product is intended for use as a positive control in Western Blot. Overexpression of the target protein was confirmed using an antibody to DDK (FLAG) epitope tag (NBP1-71705) present on the protein construct.
  
  Each vial of cell lysate contains 100ug of total protein which should be sufficient for 20-50 reactions. Depending on over-expression level, antibody affinity and detection system, some lysates can go as low as 0.1 ug per load. We recommend starting with 5ug of cell lysate. Add an equal amount of cell lysate and 2X SDS Sample buffer and boil the SDS samples for 10 minutes before loading.
• Theoretical MW: 28.2 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -80C. Avoid freeze-thaw cycles.
• Buffer: RIPA buffer

Lysate Details for Array

• Type: Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.

Alternate Names for DC-SIGNR/CD299/CLEC4M Overexpression Lysate

• CD209L
• CD209L1
• CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2
• CD299 antigenMGC129964
• CD299
• CLEC4M
• C-type lectin domain family 4, member M
• DC-SIGN2
• DC-SIGN2CD209 antigen-like protein 1
• DCSIGNR
• DC-SIGNR
• DC-SIGNRDC-SIGN-related protein
• DCSIGNRMGC47866
• Dendritic cell-specific ICAM-3-grabbing non-integrin 2
• HP10347
• liver/lymph node-specific ICAM-3 grabbing non-integrin
• Liver/lymph node-specific ICAM-3-grabbing non-integrin
• LSIGN
• L-SIGN
• LSIGNC-type lectin domain family 4 member M
• mannose binding C-type lectin DC-SIGNR

Background

This gene, often referred to as L-SIGN, is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens, with a large impact on public health, ranging from parasites to viruses. The encoded protein is a trans-membrane C-type lectin with an extracellular portion composed of a tandem repeat region and a carbohydrate recognition domain. The extracellular region of the protein plays a critical role in pathogen recognition and binding. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 30835; often referred to as DC-SIGN or CD209). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. In addition, a structural difference has been observed in the extracellular tandem-repeat region which has a constant size in DC-SIGN and is polymorphic in L-SIGN. The tandem repeat region is important for protein homo-dimerization which required for ligand-binding. Studies of the variation in tandem repeat length suggest that the region may be a target of selective pressure by infectious pathogens. [provided by RefSeq]. Transcript Variant: This variant (2) lacks a segment in the 3' UTR, as compared to variant 1. Both variants 1 and 2 encode the same isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Bioinformatics

• Gene Symbol: CLEC4M