DC-SIGNR/CD299/CLEC4M Overexpression Lysate (Native)


Western Blot: CLEC4M Overexpression Lysate (Adult Normal) [NBL1-09262] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for CLEC4M.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

DC-SIGNR/CD299/CLEC4M Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC207947 and based on accession number NM_014257. The protein contains a C-terminal DDK tag.
Homo sapiens C-type lectin domain family 4, member M (CLEC4M), transcript variant 1, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
45.2 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for DC-SIGNR/CD299/CLEC4M

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DC-SIGNR/CD299/CLEC4M Overexpression Lysate (Native)

  • CD209L
  • CD209L1
  • CD209Ldendritic cell-specific ICAM-3-grabbing nonintegrin 2
  • CD299 antigenMGC129964
  • CD299
  • CLEC4M
  • C-type lectin domain family 4, member M
  • DC-SIGN2
  • DC-SIGN2CD209 antigen-like protein 1
  • DC-SIGNRDC-SIGN-related protein
  • Dendritic cell-specific ICAM-3-grabbing non-integrin 2
  • HP10347
  • liver/lymph node-specific ICAM-3 grabbing non-integrin
  • Liver/lymph node-specific ICAM-3-grabbing non-integrin
  • L-SIGN
  • LSIGNC-type lectin domain family 4 member M
  • mannose binding C-type lectin DC-SIGNR


This gene encodes a transmembrane receptor and is often referred to as L-SIGN because of its expression in the endothelial cells of the lymph nodes and liver. The encoded protein is involved in the innate immune system and recognizes numerous evolutionarily divergent pathogens ranging from parasites to viruses, with a large impact on public health. The protein is organized into three distinct domains: an N-terminal transmembrane domain, a tandem-repeat neck domain and C-type lectin carbohydrate recognition domain. The extracellular region consisting of the C-type lectin and neck domains has a dual function as a pathogen recognition receptor and a cell adhesion receptor by binding carbohydrate ligands on the surface of microbes and endogenous cells. The neck region is important for homo-oligomerization which allows the receptor to bind multivalent ligands with high avidity. Variations in the number of 23 amino acid repeats in the neck domain of this protein are common and have a significant impact on ligand binding ability. This gene is closely related in terms of both sequence and function to a neighboring gene (GeneID 30835; often referred to as DC-SIGN or CD209). DC-SIGN and L-SIGN differ in their ligand-binding properties and distribution. Alternative splicing results in multiple variants. Transcript Variant: This variant (1) encodes the longest isoform (1). Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

Species: Hu, Mu, Pm
Applications: WB
Species: Hu, Ca, Mk
Applications: WB, IHC, IF
Species: Hu, Mu, Rt, Po, Bv
Applications: WB, ICC/IF
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Flow, IHC, IHC-Fr, IP
Species: Hu
Applications: ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
Species: Mu
Applications: Func, PAGE
Species: Hu, Mu, Rt, Ca, Rb
Applications: WB, Simple Western, ICC/IF, IHC, IHC-P
Species: Hu
Applications: Func, PAGE
Species: Hu
Applications: IHC, IHC-P
Species: Hu, Mu
Applications: WB, ELISA, ICC/IF, S-ELISA, ChIP
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
Species: Hu, Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-Fr, IHC-P
Species: Hu, Gt, Pm, Sh
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Species: Hu
Applications: WB, IHC, IHC-P

Publications for DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262) (0)

There are no publications for DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262) (0)

There are no reviews for DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262). By submitting a review earn points towards our Rewards Program.
  • 250 points for product review
  • 500 additional points for an image with your product review
  • Double points (500) if you are the first to review this product
  • Double points (1000) if you are the first to review this product with an image

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Additional DC-SIGNR/CD299/CLEC4M Products

Related Products by Gene

Bioinformatics Tool for DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262)

Discover related pathways, diseases and genes to DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262)

Discover more about diseases related to DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262).

Pathways for DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262)

View related products by pathway.

PTMs for DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262)

Learn more about PTMs related to DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262).

Research Areas for DC-SIGNR/CD299/CLEC4M Lysate (NBL1-09262)

Find related products by research area.

Blogs on DC-SIGNR/CD299/CLEC4M

There are no specific blogs for DC-SIGNR/CD299/CLEC4M, but you can read our latest blog posts.

Contact Information

Product PDFs


Gene Symbol CLEC4M

Customer Resources

Novus Review - Submit your review and earn rewards points which can be used for merchandise & discounts.
Risk Free Testing - Test on a species/application not listed above to receive a full credit towards a future purchase.

Novus' Quality Guarantee - Novus guarantees that every product we sell will work in the application and species listed on our website and datasheets.

Submit your question on NBL1-09262 below.
During business hours, we will respond to your email within 24 hours. For any questions submitted on the weekend, a response will be received on Monday.
For immediate assistance during business hours M- F (excluding major holidays), please contact us.
Ask a Scientist - We have a lab full of white coats just waiting for your scientific questions and concerns.

Customers Who Bought This Also Bought

CD4 Antibody
CD4 Antibody
CD4 Antibody