DC-SCRIPT/ZNF366 Antibody

Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with 20 ng/mL Recombinant Human IL‑4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM‑CSF (Catalog # 215-GM) for 7 days and 1 μg/mL LPS, 20 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) for last 24 hours were stained with Human DC‑SCRIPT/ZNF366 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4707, filled histogram), or control antibody (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.

• Reactivity: Hu
• Applications: WB, CyTOF-ready, Flow
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: LYOPH

DC-SCRIPT/ZNF366 Antibody Summary

• Immunogen: E. coli-derived recombinant human DC-SCRIPT/ZNF366
  Met559-Ile744
  Accession # Q8N895
• Specificity: Detects human DC-SCRIPT/ZNF366 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ZNF24 and rhZNF206 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ZNF366
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
  • Western Blot 0.1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DC-SCRIPT/ZNF366 Antibody

• DCSCRIPT
• DC-SCRIPT
• FLJ39796
• zinc finger protein 366
• ZNF366

Background

DC-SCRIPT (dendritic cell-specific transcript; also ZNF366) is an 85 kDa member of the Cys-Cys:His-His subfamily of zinc-finger proteins. This subfamily is principally involved in protein-DNA interactions. DC-SCRIPT appears to be DC specific, and is expressed in myeloid DC, plasmacytoid DC and Langerhans cells. Human DC-SCRIPT is 744 amino acids (aa) in length. It contains an R-K-R-K NLS (aa 77-80), a Pro-rich region (aa 110-219), eleven C2H2-type zinc-fingers (aa 255‑556) and an extended acidic region (aa 586-698). Over aa 559-744, human DC-SCRIPT is 68% aa identical to mouse DC-SCRIPT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DC-SCRIPT/ZNF366 Antibody (AF4707)(5)

Publications using AF4707

• Javiera Villar, Léa Ouaknin, Adeline Cros, Elodie Segura Monocytes differentiate along two alternative pathways during sterile inflammation EMBO reports 2023-07-05 [PMID: 37191947] (Western Blot, Human)
• Sondergaard Jonas Norskov, Van Heeringen Simon J, Looman Maaike W G et al. Dendritic Cells Actively Limit Interleukin-10 Production Under Inflammatory Conditions via DC-SCRIPT and Dual-Specificity Phosphatase 4. Frontiers in Immunology 1/1/2018 [PMID: 29988341]
• Hontelez , Saartje, Karthaus , Nina, Looman , Maaike W, Ansems , Marleen, Adema , Gosse J DC-SCRIPT regulates glucocorticoid receptor function and expression of its target GILZ in dendritic cells. J Immunol, 2013-02-25;190(7):3172-9. 2013-02-25 [PMID: 23440419] (Western Blot, Human)
• Hontelez S, Ansems M, Karthaus N Dendritic cell-specific transcript: dendritic cell marker and regulator of TLR-induced cytokine production. J. Immunol., 2012-05-21;189(1):138-45. 2012-05-21 [PMID: 22615205] (ICC, IHC-Fr, Western Blot, Human)
• Ansems M, Hontelez S, Looman MW, Karthaus N, Bult P, Bonenkamp JJ, Jansen JH, Sweep FC, Span PN, Adema GJ DC-SCRIPT: nuclear receptor modulation and prognostic significance in primary breast cancer. J. Natl. Cancer Inst., 2009-12-14;102(1):54-68. 2009-12-14 [PMID: 20008677] (IHC-Fr, Human)

Review for DC-SCRIPT/ZNF366 Antibody (AF4707) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer IF Human 01/08/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID 22615205
• Species: Human

Bioinformatics

• Gene Symbol: ZNF366
• Uniprot:
  • Q8N895()