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DC-SCRIPT/ZNF366 Antibody

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 (1 Review)
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Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with 20 ng/mL Recombinant Human IL‑4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM‑CSF (Catalog # 215-GM) for 7 days and 1 μg/mL LPS, 20 ng/mL Recombinant Human TNF‑ alpha (Catalog # 210-TA), and 20 ng/mL Recombinant Human IL‑1 beta /IL‑1F2 (Catalog # 201-LB) for last 24 hours were stained with Human DC‑SCRIPT/ZNF366 Antigen Affinity‑purified Polyclonal Antibody (Catalog # AF4707, filled histogram), or control antibody (Catalog # AB-108-C, open histogram), followed by Donkey Anti-Goat IgG (H+L) Phycoerythrin (Catalog # F0107). To facilitate intracellular staining, cells were fixed with paraformaldehyde and permeabilized with saponin.
Human mature dentritic cells differentated from human peripheral blood mononuclear cell derived CD14+ cells treated with 20 ng/mL Recombinant Human IL‑4 (Catalog # 204-IL) and 50 ng/mL Recombinant Human GM‑CSF ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, CyTOF-ready, Flow
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
LYOPH

Order Details

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Catalog# & Formulation Size Price
Carrier Free
AF4707-SP
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DC-SCRIPT/ZNF366 Antibody Summary

Immunogen
E. coli-derived recombinant human DC-SCRIPT/ZNF366
Met559-Ile744
Accession # Q8N895
Specificity
Detects human DC-SCRIPT/ZNF366 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) ZNF24 and rhZNF206 is observed.
Source
N/A
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
ZNF366
Purity
Immunogen affinity purified
Innovator's Reward
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Applications/Dilutions

Dilutions
  • Western Blot 0.1 ug/mL
  • CyTOF-ready
  • Intracellular Staining by Flow Cytometry 2.5 ug/10^6 cells
Reviewed Applications
Read 1 Review rated 4
using
AF4707 in the following applications:
Publications
Read Publications using
AF4707 in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Preservative
No Preservative
Concentration
LYOPH
Purity
Immunogen affinity purified
Reconstitution Instructions
Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DC-SCRIPT/ZNF366 Antibody

  • DCSCRIPT
  • DC-SCRIPT
  • FLJ39796
  • zinc finger protein 366
  • ZNF366

Background

DC-SCRIPT (dendritic cell-specific transcript; also ZNF366) is an 85 kDa member of the Cys-Cys:His-His subfamily of zinc-finger proteins. This subfamily is principally involved in protein-DNA interactions. DC-SCRIPT appears to be DC specific, and is expressed in myeloid DC, plasmacytoid DC and Langerhans cells. Human DC-SCRIPT is 744 amino acids (aa) in length. It contains an R-K-R-K NLS (aa 77-80), a Pro-rich region (aa 110-219), eleven C2H2-type zinc-fingers (aa 255‑556) and an extended acidic region (aa 586-698). Over aa 559-744, human DC-SCRIPT is 68% aa identical to mouse DC-SCRIPT.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DC-SCRIPT/ZNF366 Antibody (AF4707)(3)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC Frozen, WB.


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(2)
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(3)
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Review for DC-SCRIPT/ZNF366 Antibody (AF4707) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using AF4707:
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(1)
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(1)
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Images Ratings Applications Species Date Details
 
reviewed by:
Anonymous
 IF Human 01/08/2015
View

Summary

ApplicationImmunofluorescence
Sample TestedSee PMID 22615205
SpeciesHuman

Blocking

Blocking DetailsSee PMID 22615205

Details

Detection NotesSee PMID 22615205

Comments

CommentsPublished: See PMID 22615205Independent review from antibodyresource.com

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 Anonymous
01/08/2015
Application: IF
Species: Human
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Bioinformatics

Gene Symbol ZNF366
Uniprot