DBT Antibody (OTI1G2) [Alexa Fluor™ Plus 680]

• Reactivity: Hu, Mu, Rt
• Applications: WB, Flow, ICC/IF, CyTOF-ready
• Clone: OTI1G2
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Alexa Fluor Plus 680

DBT Antibody (OTI1G2) [Alexa Fluor™ Plus 680] Summary

• Immunogen: Full length human recombinant protein of human DBT(NP_001909) produced in HEK293T cell.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: DBT
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry
  • Immunocytochemistry/ Immunofluorescence
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Notes

This product is provided under an intellectual property license from Life Technologies Corporation. The transfer of this product is conditioned on the buyer using the purchased product solely in research conducted by the buyer, excluding contract research or any fee for service research, and the buyer must not (1) use this product or its components for (a) diagnostic, therapeutic or prophylactic purposes; (b) testing, analysis or screening services, or information in return for compensation on a per-test basis; or (c) manufacturing or quality assurance or quality control, and/or (2) sell or transfer this product or its components for resale, whether or not resold for use in research. For information on purchasing a license to this product for purposes other than as described above, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com. This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for DBT Antibody (OTI1G2) [Alexa Fluor™ Plus 680]

• BCATE2BCKAD E2 subunit
• BCKADE2
• BCKAD-E2
• branched chain acyltransferase, E2 component
• Branched-chain alpha-keto acid dehydrogenase complex component E2
• Dihydrolipoamide acetyltransferase component of branched-chain alpha-keto aciddehydrogenase complex
• dihydrolipoamide branched chain transacylase (E2 component of branched chainketo acid dehydrogenase complex; maple syrup urine disease)
• dihydrolipoamide branched chain transacylase E2
• Dihydrolipoamide branched chain transacylase
• dihydrolipoyl transacylase
• Dihydrolipoyllysine-residue (2-methylpropanoyl)transferase
• E2 component of branched chain alpha-keto acid dehydrogenase complex
• E2
• E2B
• EC 2.3.1.168
• lipoamide acyltransferase component of branched-chain alpha-keto aciddehydrogenase complex, mitochondrial
• lipoamide acyltransferase component of mitochondrial branched-chain alpha-ketoacid dehydrogenase complex
• MGC9061
• mitochondrial branched chain alpha-keto acid dehydrogenase transacylase subunit(E2b)

Background

The branched-chain alpha-keto acid dehydrogenase complex (BCKD) is an inner-mitochondrial enzyme complex involved in the breakdown of the branched-chain amino acids isoleucine, leucine, and valine. The BCKD complex is thought to be composed of a core of 24 transacylase (E2) subunits, and associated decarboxylase (E1), dehydrogenase (E3), and regulatory subunits. This gene encodes the transacylase (E2) subunit. Mutations in this gene result in maple syrup urine disease, type 2. Alternatively spliced transcript variants have been described, but their biological validity has not been determined. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DBT