DBF4 Antibody

Western Blot: DBF4 Antibody [NBP1-78282] - (1ug/ml) staining of Mouse Testis lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DBF4 Antibody Summary

• Description: Novus Biologicals Goat DBF4 Antibody (NBP1-78282) is a polyclonal antibody validated for use in WB and ELISA. Anti-DBF4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-DKKRTEYLPAHEDRT corresponding to internal region according to NP_001177646.1.
• Specificity: This antibody is expected to recognize both reported isoforms (NP_038754.1; NP_001177646.1).
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DBF4
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:16000
  • Western Blot 1 - 3 ug/ml
• Application Notes: WB: Approx. 70 kDa band observed in mouse and rat testis and thymus lysates (calculated MW of 74.2 kDa band according to NP_038754.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DBF4 Antibody

• ASKchif
• DBF4 homolog (S. cerevisiae)
• DBF4ACHIF
• DBF4-type zinc finger-containing protein 1
• ZDBF1DBF-type containing 1

Background

Regulatory subunit for CDC7 which activates its kinase activity thereby playing a central role in DNA replication and cell proliferation. Required for progression of S phase. The complex CDC7-DBF4A selectively phosphorylates MCM2 subunit at 'Ser-40' and 'Ser-53' and then is involved in regulating the initiation of DNA replication during cell cycle

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DBF4 Antibody (NBP1-78282)(1)

Publication using NBP1-78282

• Tsuji T, Lau E, Chiang GG, Jiang W. The role of Dbf4/Drf1-dependent kinase Cdc7 in DNA-damage checkpoint control. Mol Cell 2008-12-26 [PMID: 19111665]

Bioinformatics

• Gene Symbol: DBF4
• Entrez:
  • 27214(Mouse)
  • 312046(Rat)