DARPP-32 Antibody

Images

 
Western Blot: DARPP32 Antibody [NB300-304] - Detection of DARPP protein in rat hippocampal lysate using NB 300-304.
Immunohistochemistry: DARPP-32 Antibody [NB300-304] - Immunochemical staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 in green and Tuj1 in red. Cells and photo courtesy of ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, Pm, XpSpecies Glossary
Applications WB, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DARPP-32 Antibody Summary

Immunogen
Peptide corresponding to amino acid residues from the N-terminal region of rat DARPP-32, conjugated to keyhole limpet hemocyanin (KLH).
Marker
Neuronal Marker
Specificity
Specific for the ~32k DARPP-32 protein.
Predicted Species
Chicken (100%), Canine (100%), Primate (100%), Xenopus (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP1R1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Immunohistochemistry 1:250
  • Immunohistochemistry-Paraffin 1:250
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
DARPP-32 Lysate (NBL1-14679)
Publications
Read Publications using
NB300-304 in the following applications:

  • WB
    2 publications

Reactivity Notes

It is anticipated that the antibody will also react with bovine, canine, chicken, mouse, non-human primate and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for DARPP-32 Antibody

  • DARPP32
  • DARPP-32
  • PPP1R1B
  • protein phosphatase 1, regulatory (inhibitor) subunit 1B
  • regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP- 2 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DARPP-32 Antibody (NB300-304)(2)

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Video Protocols

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol PPP1R1B
Entrez
Uniprot