DARPP-32 Antibody - Azide Free

Western Blot: DARPP32 Antibody [NB300-304] - Detection of DARPP protein in rat hippocampal lysate using NB 300-304.

Immunocytochemistry/Immunofluorescence: DARPP-32 Antibody [NB300-304] - Staining of cultured CD1 mouse striatal cells showing specific labeling of DARPP-32 in green and Tuj1 in red. Cells and photo courtesy of QBMCellScience.

• Reactivity: Mu, Rt, Hu, Bv, Ca, Ch, Pm, Xp
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

DARPP-32 Antibody - Azide Free Summary

• Immunogen: Synthetic peptide corresponding to amino acid residues from the N-terminal region conjugated to KLH. Accession # Q6J4I0
• Marker: Neuronal Marker
• Specificity: Specific for endogenous levels of the ~32 kDa DARPP-32 protein.
• Predicted Species: Human (100%), Chicken (100%), Canine (100%), Xenopus (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1R1B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot 1:1000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  DARPP-32 Overexpression Lysate

Reactivity Notes

It is anticipated that the antibody will also react with bovine, canine, chicken, mouse, non-human primate and Xenopus based on the fact that these species have 100% homology with the amino acid sequence used as antigen.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for DARPP-32 Antibody - Azide Free

• DARPP32
• DARPP-32
• PPP1R1B
• protein phosphatase 1, regulatory (inhibitor) subunit 1B
• regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

DARPP-32 is a dopamine (DA) and cAMP-regulated ~32k phosphoprotein that is associated with dopaminoceptive neurons. The protein inhibits Protein Phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP- 2 is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DARPP-32 Antibody (NB300-304)(2)

Publications using NB300-304

• Hamel S, Bouchard A, Ferrario C et al. Both t-Darpp and DARPP-32 can cause resistance to trastuzumab in breast cancer cells and are frequently expressed in primary breast cancers Breast Cancer Res Treat 2010-02-01 [PMID: 19301121] (WB, Human)
• Shioda N, Han F, Moriguchi S et al. Constitutively active calcineurin mediates delayed neuronal death through Fas-ligand expression via activation of NFAT and FKHR transcriptional activities in mouse brain ischemia. J Neurochem. 2007-09-01 [PMID: 17662023] (WB, Mouse)
  
  Details:
  DARP-32 (IMG-5373): WB (mouse brain tissue), Fig 2B.

Bioinformatics

• Gene Symbol: PPP1R1B
• Entrez:
  • 84152(Human)
  • 19049(Mouse)
  • 360616(Rat)
• Uniprot:
  • Q6J4I0(Rat)