DARPP-32 [p Thr75] Antibody

Images

 
Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is ...read more
Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of Rat caudate lysates prepared from caudate slices that had been incubated in the absence and presence of okadaic acid. The labeling by the antibody to ...read more

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, Ch, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

DARPP-32 [p Thr75] Antibody Summary

Immunogen
Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH
Modification
p Thr75
Localization
Cytoplasmic
Marker
Neuronal Marker
Specificity
Specific for the ~32k DARPP-32 protein phosphorylated at Thr75. Immunolabeling is blocked by lambda-phosphatase treatment.
Predicted Species
Canine (100%), Chicken (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
PPP1R1B
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
Theoretical MW
32 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
DARPP-32 Overexpression Lysate
Publications
Read Publications using
NB300-234 in the following applications:

  • WB
    2 publications

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
Preservative
No Preservative
Purity
Immunogen affinity purified

Alternate Names for DARPP-32 [p Thr75] Antibody

  • DARPP32
  • DARPP-32
  • PPP1R1B
  • protein phosphatase 1, regulatory (inhibitor) subunit 1B
  • regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for DARPP-32 Antibody (NB300-234)(9)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 1 application: WB.


Filter By Application
WB
(2)
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Filter By Species
Human
(1)
Mouse
(1)
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Showing Publications 1 - 9 of 9.
Publications using NB300-234 Applications Species
Cunha AS, Matheus FC, Moretti M et al. Agmatine attenuates reserpine-induced oral dyskinesia in mice: Role of oxidative stress, nitric oxide and glutamate NMDA receptors. Behav. Brain Res. Jun 13 2016 [PMID: 27306571] (WB, Mouse) WB Mouse
Hamel S, Bouchard A, Ferrario C et al. Both t-Darpp and DARPP-32 can cause resistance to trastuzumab in breast cancer cells and are frequently expressed in primary breast cancers Breast Cancer Res Treat 2010 Feb [PMID: 19301121] (WB, Human) WB Human
Han et al. Spatial targeting of type II protein kinase A to filopodia mediates the regu. J. Cell Biol. 176:101.1. 2007 [PMID: 14631045]
Lindskog, M, Svenningsson, P, Pozzi, L, Kim, Y, Fienberg, AA, Bibb, JA, Fredholm, BB, Nairn, AC, Greengard, P Fisone, G. Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine,. Nature (London), 418 774 - 778. 2002 [PMID: 12181566]
Maldve, RE, Zhang, TA, Ferrani-Kile, K, Schreiber, SS, Lippmann, MJ, Snyder, GL, Feinberg, AA, Leslie, SW, Gonzales, RA, Morrisett, RA. DARPP-32 the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens, Nature Neurosci. 5 641 - 648. 2002 [PMID: 12068305]
Greengard, P. The neurobiology of slow synaptic transmission,. Science 294 1024 -1030. 2001 [PMID: 11691979]
Bibb, JA, Snyder, GL, Nishi, A, Yan, Z, Meijer, L, Fienberg, AA, Tsai, LH, Kwon, YT, Girault, JA, Czernik, AJ, Huganir, RL, Hemmings, Jr, HC, Nairn, AC, Greengard, P. Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons,. Nature (London) 402 669 - 671. 1999 [PMID: 10604473]
Fienberg, AA, Hiroi, N, Mermelstein, PG, Song, W, Snyder, GL, Nishi, A, Cheramy, A O'Callaghan, JP, Miller, DB, Cole, DG, Corbett, R, Haile, CN, Cooper, D,C, Onn, SP, Grace, AA, Ouimet, CC, White, FG, Hyman, SE, Surmeier, DG, Girault, J, Nestler, EJ, Greengard, P. DARPP-32: regulator of the efficacy of dopaminergic neurotransmission,. Science 281 838 - 842. 1998 [PMID: 9694658]
Walaas, SI Greengard, P+D1398. DARPP-32, A dopamine- adenosine 3':5'-monophosphate-regulated phosphoprotein enriched in dopamine-innervated brain regions, J. Neurosci 4 84 -98. 1984 [PMID: 6319625]

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Bioinformatics

Gene Symbol PPP1R1B
Entrez
Uniprot