DARPP-32 [p Thr75] Antibody - Azide Free

Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of rat caudate lysate showing specific immunolabeling of the ~32k DARPP-32 phosphorylated at Thr75 (Control). The phosphospecificity of this labeling is shown in the second lane (lambda-phosphatase). The blot is identical to the control except that it was incubated (1200 units for 30 min) before being exposed to the Anti-Thr75 DARPP-32. The immunolabeling is completely eliminated by treatment.

Western Blot: DARPP32 [p Thr75] Antibody [NB300-234] - Analysis of Rat caudate lysates prepared from caudate slices that had been incubated in the absence and presence of okadaic acid. The labeling by the antibody to DARPP-32 Thr75 is markedly increased by the okadaic acid treatment.

• Reactivity: Mu, Rt, Hu, Bv, Ca, Ch, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide Free

DARPP-32 [p Thr75] Antibody - Azide Free Summary

• Description: Novus Biologicals Rabbit DARPP-32 [p Thr75] Antibody - Azide Free (NB300-234) is a polyclonal antibody validated for use in WB. Anti-DARPP-32 Antibody: Cited in 9 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr75 conjugated to KLH. Accession # Q6J4I0
• Modification: p Thr75
• Localization: Cytoplasmic
• Marker: Neuronal Marker
• Specificity: Specific for endogenous levels of the ~32 kDa DARPP-32 protein phosphorylated at Thr75. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.
• Predicted Species: Human (100%), Canine (100%), Chicken (100%), Primate (100%), Bovine (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1R1B
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Theoretical MW: 32 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control:

  DARPP-32 Overexpression Lysate

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol
• Preservative: No Preservative
• Purity: Antigen Affinity-purified

Alternate Names for DARPP-32 [p Thr75] Antibody - Azide Free

• DARPP32
• DARPP-32
• PPP1R1B
• protein phosphatase 1, regulatory (inhibitor) subunit 1B
• regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DARPP-32 Antibody (NB300-234)(9)

Publications using NB300-234

• Cunha AS, Matheus FC, Moretti M et al. Agmatine attenuates reserpine-induced oral dyskinesia in mice: Role of oxidative stress, nitric oxide and glutamate NMDA receptors. Behav. Brain Res. 2016-06-13 [PMID: 27306571] (WB, Mouse)
• Hamel S, Bouchard A, Ferrario C et al. Both t-Darpp and DARPP-32 can cause resistance to trastuzumab in breast cancer cells and are frequently expressed in primary breast cancers Breast Cancer Res Treat 2010-02-01 [PMID: 19301121] (WB, Human)
• Han et al. Spatial targeting of type II protein kinase A to filopodia mediates the regu. J. Cell Biol. 176:101.1. 2007-01-01 [PMID: 14631045]
• Lindskog, M, Svenningsson, P, Pozzi, L, Kim, Y, Fienberg, AA, Bibb, JA, Fredholm, BB, Nairn, AC, Greengard, P Fisone, G. Involvement of DARPP-32 phosphorylation in the stimulant action of caffeine, . Nature (London), 418 774 - 778. 2002-01-01 [PMID: 12181566]
• Maldve, RE, Zhang, TA, Ferrani-Kile, K, Schreiber, SS, Lippmann, MJ, Snyder, GL, Feinberg, AA, Leslie, SW, Gonzales, RA, Morrisett, RA. DARPP-32 the regulation of the ethanol sensitivity of NMDA receptors in the nucleus accumbens, Nature Neurosci. 5 641 - 648. 2002-01-01 [PMID: 12068305]
• Greengard, P. The neurobiology of slow synaptic transmission, . Science 294 1024 -1030. 2001-01-01 [PMID: 11691979]
• Bibb, JA, Snyder, GL, Nishi, A, Yan, Z, Meijer, L, Fienberg, AA, Tsai, LH, Kwon, YT, Girault, JA, Czernik, AJ, Huganir, RL, Hemmings, Jr, HC, Nairn, AC, Greengard, P. Phosphorylation of DARPP-32 by Cdk5 modulates dopamine signalling in neurons, . Nature (London) 402 669 - 671. 1999-01-01 [PMID: 10604473]
• Fienberg, AA, Hiroi, N, Mermelstein, PG, Song, W, Snyder, GL, Nishi, A, Cheramy, A O'Callaghan, JP, Miller, DB, Cole, DG, Corbett, R, Haile, CN, Cooper, D,C, Onn, SP, Grace, AA, Ouimet, CC, White, FG, Hyman, SE, Surmeier, DG, Girault, J, Nestler, EJ, Greengard, P. DARPP-32: regulator of the efficacy of dopaminergic neurotransmission, . Science 281 838 - 842. 1998-01-01 [PMID: 9694658]
• Walaas, SI Greengard, P+D1398. DARPP-32, A dopamine- adenosine 3':5'-monophosphate-regulated phosphoprotein enriched in dopamine-innervated brain regions, J. Neurosci 4 84 -98. 1984-01-01 [PMID: 6319625]

Bioinformatics

• Gene Symbol: PPP1R1B
• Entrez:
  • 84152(Human)
  • 19049(Mouse)
  • 360616(Rat)
• Uniprot:
  • Q6J4I0(Rat)