DARPP-32 [p Thr34] Antibody

Western blot shows lysates of rat and mouse corpus striata tissue untreated (-) or treated (+) with calcium for 1 hour. PVDF membrane was probed with 1 µg/mL of Rabbit Anti-Mouse/Rat Phospho-DARPP-32 (T34) Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2847), followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for Phospho-DARPP-32 (T34) at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

• Reactivity: Mu, Rt
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: LYOPH

DARPP-32 [p Thr34] Antibody Summary

• Immunogen: Phosphopeptide containing mouse DARPP-32 T34 site
• Modification: p Thr34
• Specificity: Detects mouse and rat DARPP-32 when phosphorylated at T34 in Western blots. There is weak cross-reactivity with phosphorylated PP1 Inhibitor-1, which contains a nearly identical phosphorylation site. Reactivity with human Phospho-DARPP-32 has not been confirmed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: PPP1R1B
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DARPP-32 [p Thr34] Antibody

• DARPP32
• DARPP-32
• PPP1R1B
• protein phosphatase 1, regulatory (inhibitor) subunit 1B
• regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

Dopamine- and cAMP-Regulated Neuronal Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein which anomalously migrates at about 32 - 35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), it is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons. Phosphorylation of DARPP-32 at T34 has been used as an index of PKA activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DARPP-32 Antibody (AF2847)(2)

Publications using AF2847

• Gil M. Lewitus, Horia Pribiag, Rachna Duseja, Michel St-Hilaire, David Stellwagen An Adaptive Role of TNF alpha in the Regulation of Striatal Synapses The Journal of Neuroscience 4/30/2014 [PMID: 24790185]
• Metzler M, Gan L, Mazarei G Phosphorylation of huntingtin at Ser421 in YAC128 neurons is associated with protection of YAC128 neurons from NMDA-mediated excitotoxicity and is modulated by PP1 and PP2A. J. Neurosci., 2010-10-27;30(43):14318-29. 2010-10-27 [PMID: 20980587] (Western Blot, Mouse)

Bioinformatics

• Gene Symbol: PPP1R1B