DARPP-32 Antibody [Alexa Fluor® 488]

• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Alexa Fluor 488

DARPP-32 Antibody [Alexa Fluor® 488] Summary

• Immunogen: Phosphopeptide containing mouse DARPP-32 T34 site
• Specificity: Detects mouse and rat DARPP-32 when phosphorylated at T34 in Western blots. There is weak cross-reactivity with phosphorylated PP1 Inhibitor-1, which contains a nearly identical phosphorylation site. Reactivity with human Phospho-DARPP-32 has not been con
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Purity Statement: Antigen Affinity-purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Dopamine- and cAMP-Regulated Neuronal Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein which anomalously migrates at about 32 - 35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), it is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons. Phosphorylation of DARPP-32 at T34 has been used as an index of PKA activation.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.