DARPP-32 Antibody (375604) [Janelia Fluor® 549]

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clone: 375604
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Janelia Fluor 549

DARPP-32 Antibody (375604) [Janelia Fluor® 549] Summary

• Immunogen: E. coli-derived recombinant human DARPP-32
  Ala51-Thr204
  Accession # Q9UD71.2
• Specificity: Detects mouse and rat DARPP-32. Reactivity with human DARPP-32 has not been tested.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PPP1R1B
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Optimal dilution of this antibody should be experimentally determined.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

Sold under license from the Howard Hughes Medical Institute, Janelia Research Campus.

Alternate Names for DARPP-32 Antibody (375604) [Janelia Fluor® 549]

• DARPP32
• DARPP-32
• PPP1R1B
• protein phosphatase 1, regulatory (inhibitor) subunit 1B
• regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R1B