DARPP-32 Antibody (375604) [DyLight 650]

• Reactivity: Mu, Rt
• Applications: WB, IHC
• Clone: 375604
• Clonality: Monoclonal
• Host: Rat
• Conjugate: DyLight 650

DARPP-32 Antibody (375604) [DyLight 650] Summary

• Description: This conjugate is made on demand and is not held in inventory. Each order represents a unique lot. All conjugates are generated using validated conjugation protocols and meet strict degree of labeling (F/P) specifications; additional information is available upon request. The antibody concentration is provided on the individual product label.
  
  For specialized conjugation needs, like large quantities, specific concentrations, and defined F/P ratios, bulk and custom antibody conjugation services are available.
• Immunogen: E. coli-derived recombinant human DARPP-32
  Ala51-Thr204
  Accession # Q9UD71.2
• Specificity: Detects mouse and rat DARPP-32. Reactivity with human DARPP-32 has not been tested.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: PPP1R1B
• Purity: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot
• Application Notes: Recommended applications are based on validated applications from the unconjugated base product MAB4230. This conjugated antibody is not kept in inventory and is made to order using validated conjugation protocols.

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: 50mM Sodium Borate
• Preservative: 0.05% Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

Alternate Names for DARPP-32 Antibody (375604) [DyLight 650]

• DARPP32
• DARPP-32
• PPP1R1B
• protein phosphatase 1, regulatory (inhibitor) subunit 1B
• regulatory (inhibitor) subunit 1B (dopamine and cAMPregulated phosphoprotein, DARPP-32)

Background

DARPP-32, a dopamine (DA) and cAMP-regulated ~32k phosphoprotein, is associated with dopaminoceptive neurons bearing D-1 receptors in the basal ganglia. The protein inhibits protein phosphatase I when it is phosphorylated on Thr34. In contrast, when DARPP-32 is phosphorylated on Thr75 the protein acts as an inhibitor of PKA. Phosphorylation of DARPP is thought to play a critical role in the regulation of dopaminergic neurotransmission. In addition, the activity of DARPP-32 is also thought to play important roles in the actions of alcohol, caffeine and Prozac

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: PPP1R1B