DARPP-32 Antibody (375604) [Alexa Fluor® 750]

• Applications: WB, IHC
• Clone: 375604
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Alexa Fluor 750

DARPP-32 Antibody (375604) [Alexa Fluor® 750] Summary

• Immunogen: E. coli-derived recombinant human DARPP-32
  Ala51-Thr204
  Accession # Q9UD71.2
• Specificity: Detects mouse and rat DARPP-32. Reactivity with human DARPP-32 has not been tested.
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Purity Statement: Protein A or G purified

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Background

Dopamine- and cAMP-Regulated Phosphoprotein, Mr 32 kDa (DARPP-32), also known as PPP1R1B, is a 23 kilodalton protein that anomalously migrates at about
32‑35 kDa on SDS-PAGE. When phosphorylated at T34 by protein kinase A (PKA), DARPP-32 is a potent inhibitor of protein phosphatase 1 (PP1). Dephosphorylation of DARPP-32 at T34 is achieved primarily by the calcium-dependent activation of the phosphatase calcineurin. DARPP-32 is expressed almost exclusively in neuronal tissues, with highest levels in dopamine-innervated neurons.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.