DARC Antibody (358307) [Allophycocyanin]

Human red blood cells were stained with Mouse Anti-Human DARC APC-conjugated Monoclonal Antibody (Catalog # FAB4139A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 358307
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Allophycocyanin

DARC Antibody (358307) [Allophycocyanin] Summary

• Immunogen: HEK293 human embryonic kidney cell line transfected with human DARC
  Met1-Ser336
  Accession # Q16570
• Specificity: Detects human DARC. Stains human DARC transfected cells but not irrelevant transfectants.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Mouse
• Gene: ACKR1
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DARC Antibody (358307) [Allophycocyanin]

• ACKR1
• CCBP1
• CD234 antigen
• CD234
• DARC
• Dfy
• Duffy Antigen
• Duffy antigen/chemokine receptor
• Duffy blood group antigen
• Duffy blood group, chemokine receptor
• Fy Antigen
• Fy glycoprotein
• FYDuffy blood group
• Glycoprotein D
• GPD
• GPDWBCQ1
• GpFy
• Plasmodium vivax receptor

Background

Duffy Antigen Receptor for Chemokines (DARC), also known as Fy glycoprotein and CD234, is a 7TM non-signaling chemokine receptor that is expressed on venular endothelial cells and erythrocytes. DARC binds and mediates the internalization of several inflammatory CC and CXC chemokines. It transports chemokines to the lumenal face of venular endothelium to promote the extravasation of leukocytes to sites of inflammation. DARC polymorphisms are important in blood transfusion compatibility and determine the susceptibility of erythrocytes to Plasmodium vivax infection. Human DARC shares 60% amino acid sequence identity with mouse DARC.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Review for DARC Antibody (FAB4139A) (1)

Average Rating: 4

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer Flow Human 01/26/2015

Summary

• Application: Flow Cytometry
• Sample Tested: See PMID: 20040767
• Species: Human

Bioinformatics

• Gene Symbol: ACKR1
• Uniprot:
  • Q16570()