DAPK3/ZIPK Antibody

Product Discontinued

DAPK3/ZIPK Antibody Summary

• Immunogen: Rabbit anti-ZIP kinase (IN) polyclonal antibody was raised against a peptide corresponding to amino acids 279 to 298 of human origin (1).
• Specificity: ZIPK
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: DAPK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot 1:1000
• Application Notes: This polyclonal antibody can be used for detection of ZIP kinase by Western blot at 1:1000 dilution. Jurkat or HeLa whole cell lysate can be used as positive control and a 52 kDa band should be detected. It has no cross response to DAP kinase. It is human, mouse, and rat reactive.

Reactivity Notes

This antibody is reactive against Human, Mouse, Rat

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for DAPK3/ZIPK Antibody

• DAP kinase 3
• DAPK3
• DAP-like kinase
• death-associated protein kinase 3
• Dlk
• EC 2.7.11
• EC 2.7.11.1
• ZIP kinase isoform
• ZIP
• ZIPK
• ZIPKFLJ36473
• ZIP-kinase

Background

Apoptosis is mediated by death domain containing adapter molecules and a caspase family of proteases. Certain serine/threonine protein kinases, such as ASK-1 and RIP, are mediators of apoptosis. A novel serine/threonine kinase that mediates apoptosis was recently identified and designated ZIP kinase (1). ZIP kinase contains an N-terminal kinase domain and a C-terminal leucine zipper structure and binds to ATF4 that is a member of ATF/CREB family. ZIP kinase has high sequence homology to DAP kinase (death-associated protein kinase), which is a mediator of apoptosis induced by gamma interferon. Overexpression of ZIP kinase induces apoptosis. ZIP and DAP kinases represent a novel kinase family, which mediates apoptosis through their catalytic activities. The messenger RNA was ubiquitously expressed in various tissues (1).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: DAPK3
• Entrez:
  • 1613(Human)
  • 13144(Mouse)
  • 64391(Rat)