DAP3 Overexpression Lysate

Images

 
Western Blot: DAP3 Overexpression Lysate (Adult Normal) [NBP2-09807] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for DAP3.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB

Order Details

DAP3 Overexpression Lysate Summary

Immunogen
The lysate was created in HEK293T cells, using plasmid ID RC215316 and based on accession number NP_004623. The protein contains a C-terminal DDK tag.
Specificity
Homo sapiens DAP3, mRNA.
Gene
DAP3

Applications/Dilutions

Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
45.4 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -80C. Avoid freeze-thaw cycles.
Buffer
RIPA buffer

Lysate Details for Array

Type
Overexpression

Notes

HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DAP3 Overexpression Lysate

  • bMRP-10
  • DAP-3Death-associated protein 3
  • death associated protein 3
  • DKFZp686G12159,28S ribosomal protein S29, mitochondrial
  • FLJ12817
  • MGC126058
  • MGC126059
  • mitochondrial 28S ribosomal protein S29
  • MRP-S29Ionizing radiation resistance conferring protein
  • MRPS29S29mt

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB100-56116
Species: Hu, Mu, Rt, Ge
Applications: WB, ICC/IF, IHC, IHC-Fr, IHC-P, IP
NB100-56503
Species: Hu, Mu, Rt, Dr, Rb
Applications: WB, Simple Western, Flow, IB, ICC/IF, IHC, IHC-P, Flow-CS, Flow-IC
NBP1-81831
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
AF887
Species: Hu, Mu, Rt
Applications: WB, Simple Western, IHC, CyTOF-ready, ICFlow
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, Flow, GS, ICC/IF, IHC, IHC-P, IP, B/N
NBP1-91031
Species: Hu
Applications: ICC/IF, IHC, IHC-P
NB100-56618
Species: Hu, Mu
Applications: WB, Flow, ICC/IF, IHC, IHC-P, Flow-CS
AF1029
Species: Mu
Applications: WB, IHC, IP, ICC
NB100-56745
Species: Hu, Mu
Applications: WB, ChIP, ICC/IF, IP
NBP1-91684
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-81773
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP1-69210
Species: Hu, Bv, Ca, Eq, Rb
Applications: WB
NBP1-57477
Species: Hu, Mu, Rt, Bv, Ca, Eq, GP, Rb, Ye, Ze
Applications: WB, IHC

Publications for DAP3 Lysate (NBP2-09807) (0)

There are no publications for DAP3 Lysate (NBP2-09807).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for DAP3 Lysate (NBP2-09807) (0)

There are no reviews for DAP3 Lysate (NBP2-09807). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for DAP3 Lysate (NBP2-09807) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

NBP2-09807

Additional DAP3 Products

Bioinformatics Tool for DAP3 Lysate (NBP2-09807)

Discover related pathways, diseases and genes to DAP3 Lysate (NBP2-09807). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for DAP3 Lysate (NBP2-09807)

Discover more about diseases related to DAP3 Lysate (NBP2-09807).
 

Pathways for DAP3 Lysate (NBP2-09807)

View related products by pathway.

PTMs for DAP3 Lysate (NBP2-09807)

Learn more about PTMs related to DAP3 Lysate (NBP2-09807).
 

Research Areas for DAP3 Lysate (NBP2-09807)

Find related products by research area.

Blogs on DAP3

There are no specific blogs for DAP3, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our DAP3 Overexpression Lysate and receive a gift card or discount.

Bioinformatics

Gene Symbol DAP3