Recombinant Human DAO His Protein

SDS-Page: Recombinant Human DAO Protein [NBC1-28774] - 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.

• Reactivity: Hu
• Applications: PAGE
• Concentration: 0.5 mg/ml

Recombinant Human DAO His Protein Summary

• Description: A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-347 of Human DAO
  
  

  Source: E.coli

  Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL

• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: DAO
• Purity: >95%, by SDS-PAGE

Applications/Dilutions

• Dilutions:
  • SDS-Page
• Theoretical MW: 41.6 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: 20 mM Tris-HCl buffer (pH 8.0), 20% glycerol, 1 mM DTT
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: >95%, by SDS-PAGE

Alternate Names for Recombinant Human DAO His Protein

• DAAO
• D-Amino Acid Oxidase
• D-amino-acid oxidase
• DAMOX
• DAMOXOXDA
• DAO
• EC 1.4.3
• EC 1.4.3.3
• MGC35381
• OXDA

Background

D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for DAO Protein (NBC1-28774)(1)

Publication using NBC1-28774

• Huang JL, Chen XL, Guo C, Wang YX. Contributions of spinal d-amino acid oxidase to bone cancer pain Amino Acids 2012-09-21 [PMID: 22996731] (WB, Rat)

FAQs for DAO Protein (NBC1-28774). (Showing 1 - 1 of 1 FAQ).

1. I would like to find out if this enzymes DAO Protein (NBC1-28774) are functional and have been tested for enzyme activities.
   • In regards to your inquiry about our DAO recombinant protein (NBC1-28774), unfortunately this is not expected to be active and has not been tested for activity by the lab. Therefore we can neither guarantee or not guarantee that it will be functional or that would be listed as an application on the datasheet.

Bioinformatics

• Gene Symbol: DAO