Recombinant Human DAO Protein

Images

 
SDS-Page: DAO Protein [NBC1-28774] - DAAO, 41.6 kDa (367aa), confirmed by MALDI-TOF with a purity of 95% by SDS - PAGE

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
0.5 mg/ml

Order Details

Recombinant Human DAO Protein Summary

Description
A recombinant protein with a N-Terminal His-tag and corresponding to the amino acids 1-347 of Human DAO

Source: E.coli

Amino Acid Sequence: MGSSHHHHHH SSGLVPRGSH MRVVVIGAGV IGLSTALCIH ERYHSVLQPL DIKVYADRFT PLTTTDVAAG LWQPYLSDPN NPQEADWSQQ TFDYLLSHVH SPNAENLGLF LISGYNLFHE AIPDPSWKDT VLGFRKLTPR ELDMFPDYGY GWFHTSLILE GKNYLQWLTE RLTERGVKFF QRKVESFEEV AREGADVIVN CTGVWAGALQ RDPLLQPGRG QIMKVDAPWM KHFILTHDPE RGIYNSPYII PGTQTVTLGG IFQLGNWSEL NNIQDHNTIW EGCCRLEPTL KNARIIGERT GFRPVRPQIR LEREQLRTGP SNTEVIHNYG HGGYGLTIHW GCALEAAKLF GRILEEKKLS RMPPSHL

Source
E. coli
Protein/Peptide Type
Recombinant Protein
Gene
DAO
Purity
>95% pure by SDS-PAGE

Applications/Dilutions

Theoretical MW
41.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publication using
NBC1-28774 in the following applications:

  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT
Preservative
No Preservative
Concentration
0.5 mg/ml
Purity
>95% pure by SDS-PAGE

Alternate Names for Recombinant Human DAO Protein

  • DAAO
  • D-amino-acid oxidase
  • DAMOXOXDA
  • DAO
  • EC 1.4.3
  • EC 1.4.3.3
  • MGC35381

Background

D-amino-acid oxidase (DAAO) is a peroxisomal enzyme which uses flavin adenine dinucleotide (FAD) as a cofactor and oxidizes D-amino acids to the corresponding imino acids, producing ammonia and hydrogen peroxide. Its substrates include a wide variety of D-amino acids, but it is inactive on the naturally occurring L-amino acids. It has been suggested that it is involved in acid base balance in the kidney or it could act as a detoxifying agent which removes D-amino acids accumulated during aging. Recombinant human DAAO protein, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

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Publications for DAO Protein (NBC1-28774)(1)

We have publications tested in 1 confirmed species: Rat.

We have publications tested in 1 application: WB.


Filter By Application
WB
(1)
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Filter By Species
Rat
(1)
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FAQs for DAO Protein (NBC1-28774). (Showing 1 - 1 of 1 FAQ).

  1. I would like to find out if this enzymes DAO Protein (NBC1-28774) are functional and have been tested for enzyme activities.
    • In regards to your inquiry about our DAO recombinant protein (NBC1-28774), unfortunately this is not expected to be active and has not been tested for activity by the lab. Therefore we can neither guarantee or not guarantee that it will be functional or that would be listed as an application on the datasheet.

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Bioinformatics

Gene Symbol DAO