DAN/NBL1 Overexpression Lysate (Native)


Western Blot: NBL1 Overexpression Lysate (Adult Normal) [NBL1-13496] Left-Empty vector transfected control cell lysate (HEK293 cell lysate); Right -Over-expression Lysate for NBL1.

Product Details

Applications WB
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.

Order Details

DAN/NBL1 Overexpression Lysate (Native) Summary

The lysate was created in HEK293T cells, using plasmid ID RC201954 and based on accession number NM_005380. The protein contains a C-terminal DDK tag.
Homo sapiens neuroblastoma, suppression of tumorigenicity 1 (NBL1), transcript variant 2, mRNA.

Packaging, Storage & Formulations

Store at -80C. Avoid freeze-thaw cycles.
RIPA buffer


  • Western Blot
Application Notes
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Theoretical MW
17.6 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Lysate Details for DAN/NBL1

Protein State


HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.

Alternate Names for DAN/NBL1 Overexpression Lysate (Native)

  • DAN domain family member 1
  • DAND1
  • MGC8972
  • NBL1
  • neuroblastoma suppressor of tumorigenicity 1
  • neuroblastoma, suppression of tumorigenicity 1
  • Protein N03
  • suppression of tumorigenicity 1
  • Zinc finger protein DAN


This gene product is the founding member of the evolutionarily conserved CAN (Cerberus and DAN) family of proteins, which contain a domain resembling the CTCK (C-terminal cystine knot-like) motif found in a number of signaling molecules. These proteins are secreted, and act as BMP (bone morphogenetic protein) antagonists by binding to BMPs and preventing them from interacting with their receptors. They may thus play an important role during growth and development. Alternatively spliced transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]. Transcript Variant: This variant (2) has an alternate 5' exon, compared to variant 1. The encoded isoform (2) has a shorter N-terminus which is supported by counterparts of the other species. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed for 6 months from date of receipt.

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Publications for DAN/NBL1 Lysate (NBL1-13496) (0)

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Contact Information

Product PDFs


Gene Symbol NBL1

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