DACH2 Antibody

Western Blot: DACH2 Antibody [NBP1-00177] - Analysis of DACH2 in NIH3T3 cell lysate (35ug protein in RIPA buffer) using NBP1-00177 at 0.3ug/ml. Primary incubation was 1 hour. Detected by chemiluminescence.

• Reactivity: Mu, Hu
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

DACH2 Antibody Summary

• Description: Novus Biologicals Goat DACH2 Antibody (NBP1-00177) is a polyclonal antibody validated for use in WB and ELISA. Anti-DACH2 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-TRKQAVNSSRPGR corresponding to internal region according to NP_444511.1.
• Predicted Species: Human (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: DACH2
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx. 65-70 kDa band observed in NIH3T3 lysates (calculated MW of 65.3 kDa band according to human NP_444511.1 and of 68.6 kDa band according to mouse NP_291083.1).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for DACH2 Antibody

• DACH2
• dachshund homolog 2 (Drosophila)
• dachshund homolog 2
• FLJ31391
• MGC138545

Background

This gene is one of two genes which encode a protein similar to the Drosophila protein dachshund, a transcription factor involved in cell fate determination in the eye, limb and genital disc of the fly. The encoded protein contains two characteristic dachshund domains: an N-terminal domain responsible for DNA binding and a C-terminal domain responsible for protein-protein interactions. This gene is located on the X chromosome and is subject to inactivation by DNA methylation. The encoded protein may be involved in regulation of organogenesis and myogenesis, and may play a role in premature ovarian failure. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for DACH2 Antibody (NBP1-00177)(1)

Publication using NBP1-00177

• Wu K, Yang Y, Wang C et al. DACH1 inhibits transforming growth factor-beta signaling through binding Smad4. J Biol Chem 2003-12-19 [PMID: 14525983]

Bioinformatics

• Gene Symbol: DACH2
• Entrez:
  • 117154(Human)
  • 93837(Mouse)
• Uniprot:
  • NP_444511.1(Human)