DACH2 Antibody [Alexa Fluor® 700]

• Reactivity: Hu, Mu
• Applications: WB, IHC
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Alexa Fluor 700

DACH2 Antibody [Alexa Fluor® 700] Summary

• Immunogen: E. coli-derived recombinant human DACH2
  Gln419-Gln583
  Accession # Q96NX9
• Specificity: Detects human and mouse DACH2 in Western blots.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry
  • Western Blot

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied
• Buffer: Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for DACH2 Antibody [Alexa Fluor® 700]

• DACH2
• dachshund homolog 2 (Drosophila)
• dachshund homolog 2
• FLJ31391
• MGC138545

Background

DACH2 (Dachshund 2) is a 65 kDa (predicted) member of the DACH family of transcription factors. It is expressed in the dermomyotome and Mullerian duct, and promotes Mullerian duct formation and myogenin gene suppression. Human DACH2 is 599 amino acids (aa) in length and contains a DD1 domain in the N-terminus (aa 66‑162) that mediates DNA binding, and a C-terminal, coiled-coil DD2 domain (aa 452‑543) that interacts with EYA proteins. Multiple splice variants of DACH2 are known. There is an alternate start site at Met220 that may be accompanied by both a deletion of aa 259‑311 and a three aa substitution for aa 585‑599. In addition, there is a deletion of aa 163‑175 that may be accompanied by an Ala substitution for aa 584‑599. Finally, there is a 41 aa substitution for aa 1‑175. Over aa 419‑583, human DACH2 is 93% aa identical to mouse DACH2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.